FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID8_Shed_Ind1_D3_rep2_SID28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID8_Shed_Ind1_D3_rep2_SID28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68122
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT8721.2800563694548017No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC7671.1259211414814598No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT6460.9482986406740848No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA5370.7882915944922346No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA5100.7486568215848037No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA4690.688470684947594No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA4540.666451366665688No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG4230.620944775549749No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT3590.5269956842136168No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG2500.3669886380317665No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2230.32735386512433573No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2170.31854613781157337No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG2090.3068025013945568No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG1940.28478318311265083No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA1880.2759754557998884No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC1860.2730395466956343No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1820.26716772848712605No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA1730.25395613751798246No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1670.24514841020522005No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA1630.2392765919967118No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1600.23487272834033057No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1560.22900091013182233No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT1550.22753295557969527No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT1550.22753295557969527No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC1520.22312909192331404No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1520.22312909192331404No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1510.22166113737118698No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1380.20257772819353512No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1360.199641819089281No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC1350.19817386453715394No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1350.19817386453715394No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1340.19670590998502688No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA1310.19230204632864567No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG1310.19230204632864567No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1280.18789818267226446No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1280.18789818267226446No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1280.18789818267226446No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1250.18349431901588326No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1210.177622500807375No Hit
CCTCTTTGACTTGTATTCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCT1210.177622500807375No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1200.17615454625524793No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT1180.1732186371509938No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT1140.16734681894248554No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG1140.16734681894248554No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1130.16587886439035848No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1120.16441090983823142No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1110.16294295528610434No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1110.16294295528610434No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1100.16147500073397728No Hit
GTTCATTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCG1100.16147500073397728No Hit
ACCTTGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGGAGTGCC1090.16000704618185022No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC1090.16000704618185022No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG1090.16000704618185022No Hit
TGTCAAGCTTGCGGAAAAAGAAGGATTTTCAGCTGAATCAAGAAAACTGC1090.16000704618185022No Hit
CCCTTAGCAGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAG1060.155603182525469No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1040.15266727342121486No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1010.14826340976483368No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1010.14826340976483368No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1010.14826340976483368No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT990.14532750066057956No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA980.1438595461084525No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTA960.14092363700419835No Hit
ATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAAT950.1394556824520713No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT940.1379877278999442No Hit
GTTCATTGATGCTTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATAT930.13651977334781715No Hit
AAAAACAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAAT930.13651977334781715No Hit
GTATTCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGTACT920.1350518187956901No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT890.13064795513930888No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC890.13064795513930888No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT890.13064795513930888No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG870.12771204603505476No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA870.12771204603505476No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG850.12477613693080063No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC850.12477613693080063No Hit
CAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTG840.12330818237867355No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG820.12037227327441943No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA810.11890431872229237No Hit
GAAAAAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCA810.11890431872229237No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA810.11890431872229237No Hit
GCATTTTACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG790.11596840961803823No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG790.11596840961803823No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG780.11450045506591117No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT770.11303250051378408No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA760.11156454596165702No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG760.11156454596165702No Hit
GGAAACCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATG760.11156454596165702No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT750.11009659140952996No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT740.1086286368574029No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG740.1086286368574029No Hit
GATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGC740.1086286368574029No Hit
GTAAAATGCTATAAGCACAACTTTCCCTTATACTGGAGACCCTCCTTACA730.10716068230527583No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT730.10716068230527583No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC730.10716068230527583No Hit
GCTAAGGGAAAGTTGTGCTTATAGCATTTTGTGCTGGCACTTTTAAGAAA720.10569272775314877No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT710.1042247732010217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG690.10128886409676756No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA690.10128886409676756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAC151.2250169E-4145.0145
CGAATCG100.0070764627145.0145
TCGTACT151.2250169E-4145.02
GAGCGAA650.0133.846151
GAGCAAA3100.0116.9354861
CTGCTTA256.4213673E-6116.0145
AGCAGGC2000.0101.58
GCAGGCA2050.099.024399
CTGTCTT301.5887266E-596.666664145
AGCGAAA900.096.6666642
GCAAAAG3800.095.394743
GCAGGTC1000.094.259
AAGCAGG4950.090.808097
CAAAAGC4050.089.506174
GGCAGCC259.3489577E-487.0145
CTCCGAC259.3489577E-487.0145
CGAAAGC1050.082.857144
GACATAA651.5352271E-978.076921
AAAGCAG5900.076.186446
AGCCCAG300.001927978272.5145
Produced by FastQC (version 0.11.5)