FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID6_Shed_Ind2_D2_rep2_SID26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID6_Shed_Ind2_D2_rep2_SID26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154197
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG11600.7522844153907015No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT6690.43386058094515456No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA6280.4072712179873798No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA5620.36446882883583986No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA5520.3579836183583338No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG5350.34695876054657354No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT5310.3443646763555711No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC4890.3171267923500457No Hit
GAAGACAGCAGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGA3790.24578947709747917No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA3750.2431953929064768No Hit
CTTTTCTTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACC3480.22568532461721044No Hit
GCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTTCATCTTCAA3460.22438828252170925No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG3420.22179419833070682No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG3390.21984863518745504No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG3270.21206638261444777No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT3240.21012081947119596No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG3040.19715039851618382No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA3010.195204835372932No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA2880.18677406175217418No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2790.18093737232241874No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2760.1789918091791669No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2650.17185807765391026No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2520.1634273040331524No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2440.15823913565114756No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT2390.15499653041239453No Hit
GACAAGTCAAAGAGGAGTACTTGAAGATGAACAAAGGTGCTGCAATTTAT2380.1543480093646439No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2300.1491598409826391No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2220.14397167260063426No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2220.14397167260063426No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2140.13878350421862942No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2070.13424385688437518No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2060.13359533583662458No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG2050.132946814788874No Hit
GTATTCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC1970.12775864640686913No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1900.1232189990726149No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA1800.11673378859510886No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1790.11608526754735825No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1780.11543674649960765No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC1780.11543674649960765No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1780.11543674649960765No Hit
GATGTATACAGAGACGAAGCATTAAACAACCGGTTTCAGATCAAAGGTGT1750.11349118335635584No Hit
CCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTG1710.11089709916535341No Hit
GCTGTCTTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTGCTCCAG1700.1102485781176028No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1670.10830301497435099No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1630.10570893078334857No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA1590.10311484659234614No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1570.10181780449684495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA100.007085087145.0145
GAGCGAA650.0145.01
TCCCGCT100.007085087145.0145
GTCCCCG100.007085087145.0145
ATCGGGA151.2280037E-4145.0145
ATTGCAC100.007085087145.0145
AAGTGCG100.007085087145.0145
CCCTGAC256.4474043E-6116.0145
TCTCCGC256.4474043E-6116.0145
GACTCCG451.0855729E-896.66667145
GAGCAAA5650.093.672571
CCGTATC259.371585E-487.0145
CAAAAGC6100.085.573784
GCAAAAG6100.085.573783
GCAGGGT1650.083.484849
AGCGAAA1750.078.714281
AAGCAGG8150.076.5030757
AGCAGGC1150.075.652178
AGCAGGG2150.074.186048
GGCGTCT502.2969853E-672.57
Produced by FastQC (version 0.11.5)