FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID6_Shed_Ind2_D2_rep1_SID6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID6_Shed_Ind2_D2_rep1_SID6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173691
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG11470.6603681250035983No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT7710.44389173877748417No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA6440.3707733849191956No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA6400.36847044464019435No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA6320.3638645640821919No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG6190.356380008175438No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT6100.35119839254768526No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC5500.316654288362667No Hit
GCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTTCATCTTCAA4030.2320212331093724No Hit
CTTTTCTTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACC3910.22511241227236872No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT3790.2182035914353651No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG3690.21244624073786206No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA3580.20611315497060875No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA3560.20496168483110813No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA3480.20035580427310568No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3420.19690139385460387No Hit
GAAGACAGCAGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGA3420.19690139385460387No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3300.18999257301760022No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG3270.18826536780834932No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG3040.17502346120409232No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG2930.16869037543683899No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2830.16293302473933596No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2590.14911538306532868No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2590.14911538306532868No Hit
GACAAGTCAAAGAGGAGTACTTGAAGATGAACAAAGGTGCTGCAATTTAT2540.14623670771657715No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2540.14623670771657715No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2460.14163082715857472No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT2290.13184333097281956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2270.13069186083331893No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG2270.13069186083331893No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2250.12954039069381834No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2230.12838892055431772No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2200.12666171534506682No Hit
GTATTCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC2190.1260859802753165No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2190.1260859802753165No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT2160.1243587750660656No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2060.11860142436856257No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT1940.11169260353155892No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1940.11169260353155892No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1940.11169260353155892No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA1870.10766245804330679No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1870.10766245804330679No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1860.10708672297355648No Hit
GCTGTCTTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTGCTCCAG1860.10708672297355648No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1840.10593525283405587No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1810.10420804762480496No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1790.10305657748530438No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1780.10248084241555407No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA1770.10190510734580376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG100.0070858533145.0145
GGCTCCG151.2282692E-4145.0145
CGAAGCG151.2282692E-4145.0145
GAGCGAA900.0136.944441
GGACTAG203.8606202E-4108.75145
TGCCGTT203.8606202E-4108.75145
AGCAGGC1450.095.08
GAGCAAA6800.090.6251
GGCCACC259.373598E-487.0145
GCAGGGT2550.085.294129
GCAAAAG7450.081.7449653
AGACTAG1050.075.95238145
CAAAAGC8250.073.818184
TTTCCTA406.647705E-572.5145
TTATATA300.001933040272.54
CTTATAT300.001933040272.53
CAGTGTC300.001933040272.59
TATAGGC300.001933040272.57
GATAGTA300.001933040272.57
GTAGTAC300.001933040272.51
Produced by FastQC (version 0.11.5)