FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID5_Shed_Ind1_D2_rep1_SID5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID5_Shed_Ind1_D2_rep1_SID5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116828
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4380.3749101242852741No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3740.320128736261855No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3420.2927380422501455No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2490.2131338377786147No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2380.20371828671208958No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2340.20029444996062587No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT2300.19687061320916216No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2200.18831102133050295No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT2140.18317526620330743No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2060.17632759270038004No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1820.15578457219159791No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1810.15492861300373198No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1800.15407265381586605No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1720.14722498031293868No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1610.1378094292464135No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1610.1378094292464135No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1610.1378094292464135No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1590.1360975108706817No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1490.12753791899202246No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1480.12668195980415656No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1370.1172664087376314No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT1340.11469853117403361No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1340.11469853117403361No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1340.11469853117403361No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1320.11298661279830179No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1290.11041873523470402No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1250.10699489848324031No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA1240.10613893929537441No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCACG100.0070829038145.0145
GATCCCG100.0070829038145.0145
CACTGGT100.0070829038145.0145
TACTGCG100.0070829038145.0145
TCCCGCT100.0070829038145.0145
TTACGGC100.0070829038145.0145
GAGCGAA500.0130.51
GCAGGAG256.4408086E-6116.09
AGCCTGC203.8574202E-4108.75145
AGCAGGC750.0106.3333368
GCAGGGT1650.0101.060619
GCAAAAG4500.091.8333363
GAGCAAA4550.090.824181
AGCAGGG2450.088.775518
CTGCTTA259.365855E-487.0145
TGCCGCT259.365855E-487.0145
AAGCAGG5700.083.9473657
AGCCCAG353.4245553E-582.85714145
AGCGAAA851.8189894E-1276.76472
CAAAAGC5500.075.136374
Produced by FastQC (version 0.11.5)