FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID2_Shed_Ind1_D1_rep2_SID22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID2_Shed_Ind1_D1_rep2_SID22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113082
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2920.2582196989795016No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2750.24318636034028404No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2650.2343432199642737No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2600.22992164977626856No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2450.21665693921225307No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2220.1963177163474293No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2130.18835889000902No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT2020.17863143559540864No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1890.16713535310659522No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1880.1662510390689942No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1840.16271378291859007No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1660.14679613024177146No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1640.1450275021665694No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1610.14237456005376628No Hit
GATGTATACAGAGACGAAGCATTAAACAACCGGTTTCAGATCAAAGGTGT1580.1397216179409632No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1500.13264710564015492No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT1500.13264710564015492No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT1440.12734122141454873No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1360.12026670911374046No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1340.1184980810385384No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA1330.11761376700093738No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT1310.11584513892573531No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG1310.11584513892573531No Hit
ATACATGATAGAATCCCTCATCGAACCCTATTAATGAATGAGTTGGGTGT1290.11407651085053325No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA1210.10700199854972497No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC1200.10611768451212396No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT1190.10523337047452291No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1180.10434905643692188No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC1180.10434905643692188No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT1170.10346474239932085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1160.10258042836171981No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT1140.10081180028651773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAA100.0070826053145.06
GTGGCTT100.0070826053145.0145
GAGCGAA151.2271437E-4145.01
TCCAACG100.0070826053145.0145
CTCTAAG100.0070826053145.0145
AGCGAAA900.0112.777781
CTTATCG203.8570963E-4108.755
TCGTACT203.8570963E-4108.752
CATTTCG301.5932997E-596.666664145
TGCCGCT301.5932997E-596.666664145
TCGTTCA259.365071E-487.09
AGCCCAG259.365071E-487.0145
CCCACCC259.365071E-487.0145
CGAAAGC1250.081.23
AAGTAAA300.001931288472.52
TAAACTA502.2930144E-672.55
CTAGTAG502.2930144E-672.53
GAAGTAA300.001931288472.51
CCCTTTA300.001931288472.5145
TTCATTC300.001931288472.5145
Produced by FastQC (version 0.11.5)