FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID2_Shed_Ind1_D1_rep1_SID2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID2_Shed_Ind1_D1_rep1_SID2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135297
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3510.2594292556375973No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2930.21656060370887753No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2820.2084303421361893No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2450.18108309866441977No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2370.17517018115701014No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2250.1663008048958957No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2230.16482257551904328No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2200.16260523145376468No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2180.16112700207691227No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2140.15817054332320746No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2050.15151851112737164No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1930.1426491348662572No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1930.1426491348662572No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1910.1411709054894048No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1910.1411709054894048No Hit
ATCATGTATTGTGTCATTTGAATGTTTGTTGTCTAGTGTGGTCCCCTGCC1810.13377975860514277No Hit
ATACATGATAGAATCCCTCATCGAACCCTATTAATGAATGAGTTGGGTGT1760.13008418516301173No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1680.12417126765560212No Hit
GTATTGGTTCATGGTCTCAAAATATCCTCAGAACCCAGGAGTCGGAATGC1670.12343215296717591No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG1670.12343215296717591No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1660.12269303827874972No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1650.1219539235903235No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1630.12047569421347111No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1560.11530189139448768No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1550.11456277670606149No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1450.10717162982179945No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1420.10495428575652084No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1410.10421517106809465No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1390.10273694169124223No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAGTG100.007084132145.0145
GAGCGAA301.096505E-7120.8333361
GCAGGAG203.858753E-4108.759
CTGCTTA353.426519E-582.85714145
CGAAAGC1050.075.952383
CGGCCTG406.6423905E-572.5145
AGCCCCG300.001932112672.5145
AGCGAAA1100.072.51
TCGTACT300.001932112672.52
AGGGTCA451.1901431E-464.444445
CAAGGGT1250.063.84
AGCCCAG350.00355984162.142853145
AGTGGGG350.00355984162.142853145
CTAACAT350.00355984162.1428536
AACATCG350.00355984162.1428538
TGCCGTT350.00355984162.142853145
CTGTCTT606.7739293E-660.416668145
TATCCCG606.7739293E-660.4166689
GCAGGGT1102.5465852E-1159.318189
AGGGTGT1007.1122486E-1058.06
Produced by FastQC (version 0.11.5)