FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID24_NL_Cnt5_D3_rep2_SID39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID24_NL_Cnt5_D3_rep2_SID39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154565
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3930.25426196098728693No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3900.2523210299873839No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT3360.2173842719891308No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC3200.20703263998964835No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3100.20056286998997186No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2560.1656261119917187No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2300.14880470999255976No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2230.14427587099278621No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2180.14104098599294795No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2130.1378061009931097No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2100.13586516999320675No Hit
TTCAATGGGAATACAGAGGGAAGAACATCTGACATGAGGACCGAAATCAT2080.13457121599327143No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2070.1339242389933038No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG2050.13263028499336849No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2010.13004237699349788No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1990.1287484229935626No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1970.12745446899362728No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1930.12486656099375668No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1900.1229256299938537No Hit
CCCACTGAATGCTGCCATAATGGTTGTTCTGTCAAAAGGGAGATTTCTCT1810.11710283699414485No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT1810.11710283699414485No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1740.11257399799437129No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1740.11257399799437129No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1710.11063306699446834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1700.1099860899945007No Hit
TTCAAAATGAGTTTAACAAGGCATGCGAACTGACAGATTCAAGCTGGATA1650.10675120499466244No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1550.10028143499498593No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG100.0070851045145.0145
TTCGGTA100.0070851045145.0145
CTCTGCG100.0070851045145.0145
CGAAGCG100.0070851045145.0145
GAGCGAA351.891749E-9124.285711
GCAGGAG203.8598076E-4108.759
TTCTGCG203.8598076E-4108.75145
TCTCCGC259.37163E-487.0145
CTCTTTA353.4280696E-582.85714145
GCAAAAG4000.074.31253
AGCGAAA607.986819E-872.52
GAGCAAA4750.065.631581
GCAGGGT1009.094947E-1265.259
CAAAAGC4700.063.2446824
AAGCAGG5350.062.336457
CAAGGTA350.003560801762.1428574
CGAAAGC753.740297E-758.0000044
ATCTAGG400.006041249354.3751
TCTAGGA400.006041249354.3752
CAAGGTC400.006041249354.3758
Produced by FastQC (version 0.11.5)