FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID24_NL_Cnt5_D3_rep1_SID19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID24_NL_Cnt5_D3_rep1_SID19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109627
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3890.3548395924361699No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3480.317440046703823No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3130.2855136052249902No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2940.26818210842219525No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG2340.21345106588705337No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2270.20706577759128683No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT2180.1988561212110155No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC2130.1942952009997537No Hit
TTCAATGGGAATACAGAGGGAAGAACATCTGACATGAGGACCGAAATCAT1870.17057841590119222No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1770.16145657547866857No Hit
CCCACTGAATGCTGCCATAATGGTTGTTCTGTCAAAAGGGAGATTTCTCT1750.15963220739416384No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1730.1578078393096591No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1730.1578078393096591No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1690.15415910314064965No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1600.14594944676037838No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1430.13044231804208817No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1430.13044231804208817No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT1430.13044231804208817No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1420.12953013399983582No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1370.124969213788574No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1360.12405702974632163No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1340.12223266166181687No Hit
TTCAAAATGAGTTTAACAAGGCATGCGAACTGACAGATTCAAGCTGGATA1330.12132047761956452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1260.11493518932379797No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1250.11402300528154562No Hit
ATCATGTATTGTGTCATTTGAATGTTTGTTGTCTAGTGTGGTCCCCTGCC1230.11219863719704089No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA1220.1112864531547885No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1190.10854990102803141No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1180.10763771698577905No Hit
ATACATGATAGAATCCCTCATCGAACCCTATTAATGAATGAGTTGGGTGT1160.10581334890127432No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1110.10125242869001251No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1100.10034024464776012No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTA151.2270424E-4145.0145
GGCAGCC151.2270424E-4145.0145
CAGCCGA100.0070823114145.0145
CGAGACG151.2270424E-4145.0145
GGTTCTA100.0070823114145.0145
GGTTATA100.0070823114145.0145
CTCTAGG100.0070823114145.0145
GAGCGAA259.3643036E-487.01
AGCAGGC502.2497261E-887.08
TTCGTAA353.4236105E-582.85714145
GAGCAAA3950.082.594941
GCAAAAG4050.080.555563
TCGCCAT300.001931130972.5145
CGAAAGC607.964991E-872.53
AGCCCCG300.001931130972.5145
AGCGAAA651.3864701E-766.923071
AAGCAGG5300.064.292457
GCAGGGT1700.063.970599
GTAGTAA1051.6370905E-1162.1428571
TAGTAAA702.3152461E-762.1428572
Produced by FastQC (version 0.11.5)