FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID21_NL_Cnt2_D3_rep2_SID36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID21_NL_Cnt2_D3_rep2_SID36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57884
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1990.34379103033653513No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1530.26432174694216015No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1340.23149747771404877No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1200.2073111740722825No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1100.19003524289959228No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA970.167576532375095No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT930.16066615990601893No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC840.14511782185059774No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT820.1416626356160597No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA800.13820744938152166No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA790.13647985626425266No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT760.13129707691244558No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT760.13129707691244558No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT720.12438670444336951No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT710.12265911132610047No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG710.12265911132610047No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC700.12093151820883145No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA670.11574873885702439No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA650.11229355262248635No Hit
GATGTATACAGAGACGAAGCATTAAACAACCGGTTTCAGATCAAAGGTGT610.10538318015341026No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA610.10538318015341026No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA600.10365558703614125No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA580.1002004008016032No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC100.0070737256145.06
AGCCACG100.0070737256145.0145
CCCCCGG100.0070737256145.0145
GAGCGAA151.2240701E-4144.999981
AACTGCG151.2240701E-4144.99998145
GCAGGGT600.0132.916669
TTAGGCA203.847469E-4108.754
CGGTCCA203.847469E-4108.759
CACAATC203.847469E-4108.753
CTCAGCG203.847469E-4108.754
GCAAAAG1600.090.6253
CAGCGGT259.3417836E-487.06
GCGGTCC259.3417836E-487.08
AGCAGGG1300.083.653858
AGCAGGC353.4099176E-582.857148
GAGCAAA1800.080.555561
GTATATA502.27885E-672.51
TCGGGGC300.001926504572.49999145
AGCGAAA300.001926504572.499991
TAGGCAG300.001926504572.499995
Produced by FastQC (version 0.11.5)