FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID21_NL_Cnt2_D3_rep1_SID16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID21_NL_Cnt2_D3_rep1_SID16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119122
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3980.33411124729269154No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2820.23673208979029903No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2820.23673208979029903No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2710.22749785933748592No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2190.18384513356055138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2060.17293195211631773No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1900.15950034418495324No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1850.15530296670640184No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1720.1443897852621682No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1710.1435503097664579No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1680.14103188327932709No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1610.13515555480935512No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1600.13431607931364484No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1590.13347660381793455No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1520.1276002753479626No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1490.12508184886083176No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1490.12508184886083176No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1460.12256342237370091No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1460.12256342237370091No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1280.10745286345091587No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1280.10745286345091587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTA151.2273077E-4145.0145
TTTCCTA100.007083077145.0145
TCGATTC100.007083077145.0145
GAGCGAA253.6910933E-8145.01
AGGTAGT301.5936525E-596.6666646
GGTAGTT301.5936525E-596.6666647
GCAGGGT1650.092.2727369
GAGCAAA3750.083.133341
CAAGGTA353.424834E-582.857144
GCAAAAG3700.082.29733
TCGTACT554.361209E-879.0909042
TTAGATA300.001931543772.53
AAGCAGG4800.072.57
TGTTAAC300.001931543772.5145
CGAGACG502.293771E-672.5145
ACAAGGG809.822543E-1172.53
AGCAGGG2700.069.814818
AGGGTGT955.456968E-1268.684216
AAGGGTG851.6734703E-1068.23535
AGCGAAA554.0396953E-665.909092
Produced by FastQC (version 0.11.5)