FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID1_SV_IAV_X31_D0_rep1_SID1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID1_SV_IAV_X31_D0_rep1_SID1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103605
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG6760.6524781622508566No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA5770.556922928430095No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT3840.37063848269871147No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT3710.35809082573234885No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2830.2731528401138941No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2470.2384054823608899No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2450.23647507359683415No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2420.23357946045075043No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT2320.2239274166304715No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC2240.21620578157424833No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2170.20944935090005307No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2050.19786689831571835No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2040.19690169393369047No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1980.1911104676415231No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1900.18338883258529995No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1790.1727715843829931No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1720.16601515370879782No Hit
GAGCAAAAGCAGGAGTGAAAATGAATCCAAATCAAAAGATAATAACAATT1700.16408474494474204No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1590.1534674967424352No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1590.1534674967424352No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1560.15057188359635154No Hit
ATCATGTATTGTGTCATTTGAATGTTTGTTGTCTAGTGTGGTCCCCTGCC1440.13898943101201677No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1440.13898943101201677No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1440.13898943101201677No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1420.13705902224796102No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1420.13705902224796102No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1410.1360938178659331No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1370.13223300033782154No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1370.13223300033782154No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1350.13030259157376575No Hit
ATACATGATAGAATCCCTCATCGAACCCTATTAATGAATGAGTTGGGTGT1350.13030259157376575No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1330.12837218280970997No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1330.12837218280970997No Hit
GTATTGGTTCATGGTCTCAAAATATCCTCAGAACCCAGGAGTCGGAATGC1280.12354616089957048No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC1200.11582452584334732No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA1200.11582452584334732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170.11292891269726366No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT1140.11003329955117996No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1120.10810289078712416No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1120.10810289078712416No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT1100.10617248202306839No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC1090.1052072776410405No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA1050.10134646011292892No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT1040.10038125573090101No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1040.10038125573090101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGT151.2268499E-4145.0145
GGACTAG100.007081756145.0145
AATATAT100.007081756145.0145
GATAGTA100.007081756145.07
TAGTATA100.007081756145.09
GCAGGAG450.0128.888899
GAGCGAA950.0122.105271
CGAAAAC203.8561752E-4108.75145
TGCCGTT203.8561752E-4108.75145
AGCAGGG2800.093.214298
GCAGGGT2350.092.553199
GAGCAAA5100.092.4019551
TAAACTA259.362843E-487.05
TAACAAC259.362843E-487.04
AGCAGGC502.2484528E-887.08
GCAAAAG5450.086.467893
AAGCAGG6850.085.729937
AGCGAAA1400.082.857142
CAAAAGC5850.080.555564
TCACCTT300.001930831272.5145
Produced by FastQC (version 0.11.5)