FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID19_NL_Ind3_D3_rep2_SID34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID19_NL_Ind3_D3_rep2_SID34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194191
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT28041.4439392144847083No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT13340.6869525364203284No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC13020.6704739148570222No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT12890.6637794748469291No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA8930.4598565330010145No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA7980.4109356252349491No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT7330.3774634251844833No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT6990.35995488977347045No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT6900.3553202774587906No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG6890.3548053205349373No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATT6770.3486258374486974No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA5720.29455536044409886No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT5600.288375877357859No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA5320.27395708348996606No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATTTGAAA5130.26417290193675297No Hit
CCTTACAGCCATGGGACAGGAACAGGATATTTGAAAAATTCTTCCCCAGC5100.262628031165193No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG4800.24717932344959342No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG4470.2301857449624339No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGA4450.22915583111472723No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC4260.2193716495615142No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG3970.20443789876976792No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3920.2018631141505013No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA3820.19671354491196813No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA3780.19465371721655483No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGA3570.18383962181563512No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT3540.18229475104407517No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT3520.18126483719636852No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC3460.17817509565324863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3280.16890587102388885No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT3260.1678759571761822No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC3260.1678759571761822No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA3220.16581612948076893No Hit
TCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTG3090.1591216894706758No Hit
GTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTT3090.1591216894706758No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA3070.15809177562296914No Hit
GTATAAGGGAAAGTTGTGCTTATAGCATTTTGTGCTGGCACTTTTAAGAA3040.15654690485140918No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATATCCTGTTCCTGTCCCATG2950.1519122925367293No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT2930.15088237868902266No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2820.14521785252663613No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2780.14315802483122286No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG2660.13697854174498303No Hit
TTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATC2620.13491871404956976No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG2580.13285888635415646No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2540.1307990586587432No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2520.12976914481103655No Hit
ATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCACAAAATGCTATA2520.12976914481103655No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA2500.1287392309633299No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2490.1282242740394766No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2480.12770931711562328No Hit
AGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGAT2430.12513453249635667No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTA2430.12513453249635667No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT2390.1230747048009434No Hit
CAAATATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG2380.12255974787709009No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2340.1204999201816768No Hit
CCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAAT2340.1204999201816768No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA2310.11895504941011684No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA2300.11844009248626353No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2290.1179251355624102No Hit
AGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGT2260.11638026479085024No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2250.11586530786699693No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA2240.1153503509431436No Hit
AAATCAATCCGTGCATCAATTCGGGCTCTGGAAACCATAGCCTCCACCAT2200.11329052324773033No Hit
GCAATAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGGA2180.11226060940002368No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2180.11226060940002368No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT2180.11226060940002368No Hit
CCATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG2160.11123069555231706No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2150.11071573862846372No Hit
GCTGTAAGGAGGGTCTCCAGTATAAGGGAAAGTTGTGCTTATAGCATTTT2150.11071573862846372No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2140.11020078170461041No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2130.1096858247807571No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG2130.1096858247807571No Hit
AAGCACAACTTTCCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAG2120.10917086785690376No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG2120.10917086785690376No Hit
GATTTTCGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAATGATC2110.10865591093305045No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2060.10608112631378386No Hit
ATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATCTTCATGATCTCA2060.10608112631378386No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2060.10608112631378386No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT2050.10556616938993052No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2030.1045362555422239No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA2000.10299138477066394No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA1990.1024764278468106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGCA100.0070864935145.04
TATGCGC100.0070864935145.0145
GTCAAAC100.0070864935145.0145
CTGTCCT256.451657E-6116.0145
CACGAGG256.451657E-6116.0145
CCGTATC256.451657E-6116.0145
GAGCGAA750.0116.01
AACTGCG256.451657E-6116.0145
GAGCAAA8100.0107.407411
AGCAGGC4600.0104.021748
GCAAAAG8950.096.3966453
AGCGAAA1850.094.054051
CCCCTAC259.375277E-487.0145
TATAGGC259.375277E-487.07
CAAAAGC9950.086.708544
AAGCAGG11500.082.586967
TTAGTAG451.229133E-680.555562
CGAAAGC2300.075.6521763
ATTAGTA502.2992372E-672.51
AATTTAC502.2992372E-672.5145
Produced by FastQC (version 0.11.5)