FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID19_NL_Ind3_D3_rep1_SID14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID19_NL_Ind3_D3_rep1_SID14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112769
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT18741.6618042192446507No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT8480.7519797107361065No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC7050.6251718114020697No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT5690.504571291755713No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA4970.44072395782528884No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA4590.40702675380645387No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT4010.35559417925139No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG3940.3493867995637099No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT3470.3077086788035719No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT3260.2890865397405315No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGA3250.2881997712137201No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG3080.27312470625792545No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATT2900.25716287277531946No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2830.25095549308763937No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2770.2456348819267707No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2590.22967304844416464No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA2490.22080536317605018No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATTTGAAA2310.20484352969344413No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGA2270.20129645558619835No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2240.198636150005764No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2150.19065523326446096No Hit
CCTTACAGCCATGGGACAGGAACAGGATATTTGAAAAATTCTTCCCCAGC2150.19065523326446096No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2110.18710815915721518No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC2060.18267431652315796No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG1910.16937278862098626No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1870.16582571451374048No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1810.1605051033528718No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG1800.15961833482606033No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1750.15518449219200312No Hit
GCAATAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGGA1720.15252418661156877No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1710.15163741808475734No Hit
CCATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG1700.15075064955794587No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1660.1472035754507001No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1580.14010942723620853No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1580.14010942723620853No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1570.13922265870939707No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1490.13212851049490554No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT1490.13212851049490554No Hit
CAAATATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG1470.13035497344128263No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA1470.13035497344128263No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1460.1294682049144712No Hit
TCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTG1440.1276946678608483No Hit
GATTTTCGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAATGATC1430.12680789933403686No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA1420.1259211308072254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1420.1259211308072254No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC1370.12148728817316817No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1360.12060051964635672No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1320.11705344553911093No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1310.11616667701229949No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTA1300.11527990848548804No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT1270.1126196029050537No Hit
CCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAAT1260.11173283437824226No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1250.11084606585143081No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1240.10995929732461937No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT1230.10907252879780792No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1210.10729899174418503No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1210.10729899174418503No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1200.10641222321737358No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA1190.10552545469056213No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1170.10375191763693924No Hit
AAGCACAACTTTCCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAG1170.10375191763693924No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA1170.10375191763693924No Hit
GTATAAGGGAAAGTTGTGCTTATAGCATTTTGTGCTGGCACTTTTAAGAA1160.10286514911012778No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT1150.10197838058331635No Hit
ATCATGTATTGTGTCATTTGAATGTTTGTTGTCTAGTGTGGTCCCCTGCC1150.10197838058331635No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT1140.1010916120565049No Hit
GAGCAAAAGCAGGAGTGAAAATGAATCCAAATCAAAAGATAATAACAATT1140.1010916120565049No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTAC151.2271354E-4145.0145
AACTGCG202.1223314E-6145.0145
GAGCGAA650.0111.538471
GAGCAAA4550.0109.945061
TTAGTAG203.8570692E-4108.751
AGCAGGC3000.099.0833368
GCAAAAG5450.093.119273
AGTAGAA2150.080.930231
AAGCAGG7200.079.5486157
CAAAAGC6400.079.2968754
GCAGGCA3800.078.223699
CTGCTTA406.637599E-572.5145
GTAGAAA2400.072.52
TCGTATC300.001931275472.5145
GGGCCCC406.637599E-572.5145
GTATATA809.822543E-1172.54
CGAAAGC1350.069.814823
AGCGAAA1350.069.814821
TATAGGT851.6734703E-1068.23537
AGAAACA2650.065.660384
Produced by FastQC (version 0.11.5)