FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID18_NL_Ind2_D3_rep1_SID13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID18_NL_Ind2_D3_rep1_SID13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119233
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG16361.372103360646801No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT12671.0626252799141178No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA6470.5426350087643522No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT6070.509087249335335No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC4390.36818665973346304No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3780.3170263266042119No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG3730.31283285667558475No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT3550.2977363649325271No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA3500.2935428950038999No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA3170.26586599347496076No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT3140.26334991151778453No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA3060.25664035963198106No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA3040.2549629716605302No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2970.2490921137604522No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2870.24070517390319796No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG2860.2398664799174725No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG2720.2281247641173165No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT2510.21051219041708252No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG2430.20380263853127908No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2330.1954156986740248No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2240.18786745280249595No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC2070.17360965504516365No Hit
GTATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCCCATTGGA2050.1719322670737128No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2030.17025487910226195No Hit
GTACTCATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGAT1940.16270663323073312No Hit
GAACAAATGTACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAG1820.15264230540202797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1820.15264230540202797No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT1750.14677144750194995No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1750.14677144750194995No Hit
GTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTGCTCCAGTTTCGG1720.14425536554477367No Hit
CCTTTGTTCATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCA1700.14257797757332283No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG1690.14173928358759738No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1680.14090058960187196No Hit
CATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGG1660.1392232016304211No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA1650.13838450764469568No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG1620.13586842568751942No Hit
CATGTGGAGTTGATAATATGCACTCACTTGGAAGTATGCTTCATGTATTC1600.13419103771606855No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1560.13083626177316682No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1510.1266427918445397No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1510.1266427918445397No Hit
CCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTG1510.1266427918445397No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1510.1266427918445397No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA1500.12580409785881425No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1480.1241267098873634No Hit
CCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAAT1430.11993323995873625No Hit
CAAACAAATTTGCAGCAATATGCACTCACTTGGAAGTATGCTTCATGTAT1380.11573977003010912No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTT1360.11406238205865825No Hit
GTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGCACCGC1330.11154630010148196No Hit
GGAAACCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATG1330.11154630010148196No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1320.11070760611575653No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA1320.11070760611575653No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA1290.10819152415858024No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1280.10735283017285482No Hit
GAGCAAAAGCAGGAGTGAAAATGAATCCAAATCAAAAGATAATAACAATT1280.10735283017285482No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT1240.10399805422995313No Hit
GCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTTCATCTTCAA1220.10232066625850227No Hit
GTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGGAA1220.10232066625850227No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTATCCTTCCAGATTCGA1220.10232066625850227No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1210.10148197227277683No Hit
GGATAAAGAAAGAAGAGTTCACTGAGATCATGAAGATCTGTTCCACCATT1200.10064327828705141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGTC100.0070830854145.08
TGCCGTT351.888111E-9124.28571145
CCGTACA301.09570465E-7120.833336145
GAGCGAA600.0108.751
GAGCAAA4550.0106.758251
AGCAGGC2400.093.6458368
CTGTCCT259.3663315E-487.0145
GCAAAAG5800.083.753
AGCGAAA1350.080.555561
CAAAAGC6100.079.631144
AGCAGGG1900.076.315798
AAGCAGG7300.075.4794547
CGAAAGC1450.075.03
CGCCTTT406.639158E-572.5145
GCAGGCA3150.071.3492059
GCAGGGT1750.070.428579
TATATAG451.1895652E-464.444445
AGCCCAG350.003558803162.142857145
GGATACG350.003558803162.142857145
GTATGCG702.3169741E-762.142857145
Produced by FastQC (version 0.11.5)