FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID17_NL_Ind1_D3_rep1_SID12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID17_NL_Ind1_D3_rep1_SID12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66920
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT6570.9817692767483562No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT6530.9757919904363419No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC4530.6769276748356247No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA3560.5319784817692768No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2920.4363419007770472No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2440.3646144650328751No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1830.2734608487746563No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1610.2405857740585774No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1600.23909145248057379No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT1510.22564255827854152No Hit
GGAGAATACAAGTCAAAGAGGAGTACTTGAAGATGAACAAATGTACCAAA1480.22115959354453077No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA1470.21966527196652721No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT1450.21667662881052005No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1350.20173341303048414No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA1340.2002390914524806No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC1330.19874476987447698No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA1290.19276748356246265No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1270.18977884040645546No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG1200.17931858936043038No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1160.17334130304841602No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1160.17334130304841602No Hit
GTATTCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGTACT1150.17184698147041244No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1070.15989240884638375No Hit
GCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGTACATTTGTT1070.15989240884638375No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG1040.15540944411237298No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1000.14943215780035865No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT980.14644351464435146No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT950.1419605499103407No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT900.13448894202032277No Hit
CTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTC880.1315002988643156No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG880.1315002988643156No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT870.13000597728631202No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG870.13000597728631202No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT840.12552301255230125No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC840.12552301255230125No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC810.12104004781829049No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT810.12104004781829049No Hit
CCTCTTTGACTTGTATTCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCT790.11805140466228334No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA780.11655708308427974No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA780.11655708308427974No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA770.11506276150627616No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA770.11506276150627616No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC750.11207411835026898No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA750.11207411835026898No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG730.1090854751942618No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA730.1090854751942618No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA730.1090854751942618No Hit
GGTTCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAAGCATACTT720.10759115361625822No Hit
GCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTT720.10759115361625822No Hit
ACCTTGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGGAGTGCC710.10609683203825462No Hit
GAAAATGCAGTGATGATGCCAGCACATGGTCCAGCCAAAAACATGGAGTA700.10460251046025104No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG700.10460251046025104No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT700.10460251046025104No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT690.10310818888224747No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA690.10310818888224747No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA680.10161386730424388No Hit
GTACTGATGTGTCCTGTTGACAGTATCCATGGTGTATCCTGTTCCTGTCC680.10161386730424388No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC670.1001195457262403No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA670.1001195457262403No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG100.007076185145.0145
GAGCGAA650.0145.01
CGTCCTG100.007076185145.0145
ACCCCCC100.007076185145.0145
ACCTTGG151.2249209E-4145.01
AAGGGTG301.0903932E-7120.8333365
GAGCAAA2950.0117.96611
CTGTCTT203.850135E-4108.75145
TAAACTA203.850135E-4108.755
ATTCAGT203.850135E-4108.75145
GCAGGGT700.093.214299
AGGGTGT406.0549064E-790.6256
ACAAGGG406.0549064E-790.6253
GCAAAAG3850.088.506493
GTGTCGC259.3482324E-487.09
CTATTCG259.3482324E-487.09
CAAAAGC3950.086.265824
CTCCGAC353.4138375E-582.85714145
AAGCAGG5200.082.259627
AAAGCAG5550.077.0720756
Produced by FastQC (version 0.11.5)