000000000-BTF83_l01_n01_Exp1_SrcID12_Shed_Cnt2_D3_rep2

Basic Statistics

Measure	Value
Filename	000000000-BTF83_l01_n01_Exp1_SrcID12_Shed_Cnt2_D3_rep2_SID31.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60407
Sequences flagged as poor quality	0
Sequence length	151
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	534	0.8840035095270417	No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT	114	0.18871985034846955	No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA	109	0.18044266392967703	No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA	97	0.16057741652457497	No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA	96	0.15892197924081647	No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA	95	0.15726654195705797	No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA	88	0.14567848097074843	No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT	81	0.13409041998443888	No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT	81	0.13409041998443888	No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT	80	0.13243498270068038	No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC	80	0.13243498270068038	No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG	80	0.13243498270068038	No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT	72	0.11919148443061234	No Hit
GTACAGAGAAATCTCCCTTTTGACAGAACAACCATTATGGCAGCATTCAA	69	0.11422517257933683	No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG	69	0.11422517257933683	No Hit
GTAGTAATGACTGATGGAAGTGCTTCAGGAAGAGCCGATACTAGAATACT	68	0.11256973529557833	No Hit
ATACATGATAGAATCCCTCATCGAACCCTATTAATGAATGAGTTGGGTGT	66	0.10925886072806132	No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC	66	0.10925886072806132	No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA	66	0.10925886072806132	No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT	64	0.10594798616054431	No Hit
GTATTGGTTCATGGTCTCAAAATATCCTCAGAACCCAGGAGTCGGAATGC	63	0.10429254887678581	No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG	62	0.10263711159302731	No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT	61	0.10098167430926878	No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA	61	0.10098167430926878	No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT	61	0.10098167430926878	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACTAG	10	0.007074494	145.0	1
ACTAGAC	30	0.0019269178	72.5	3
AAGGGTG	30	0.0019269178	72.5	5
AAGGGTA	40	6.612668E-5	72.5	5
ACAAGGG	55	4.0156265E-6	65.90909	3
AATATTG	35	0.003550305	62.142857	3
ATACTAA	35	0.003550305	62.142857	3
AACATCG	35	0.003550305	62.142857	8
AGCGAAA	35	0.003550305	62.142857	1
ACATGTA	35	0.003550305	62.142857	6
CAAGGGT	60	6.72874E-6	60.416668	4
TAATATT	40	0.0060235052	54.375	2
GACAGCT	40	0.0060235052	54.375	7
CTCCGAC	40	0.0060235052	54.375	145
CAACATG	40	0.0060235052	54.375	4
AGGGTGT	40	0.0060235052	54.375	6
TCCTATG	40	0.0060235052	54.375	7
GCCTAAG	40	0.0060235052	54.375	1
AGTAGAA	95	3.0739102E-8	53.421055	1
GTAGAAA	100	4.6053174E-8	50.75	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content