FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID10_Shed_Ind3_D3_rep2_SID30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID10_Shed_Ind3_D3_rep2_SID30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142094
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT15201.069714414401734No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT7350.5172632201218911No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC6790.4778526890649852No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT5550.39058651315326476No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA4690.33006319760158764No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA4680.32935943811842866No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG4290.30191281827522626No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGA3300.23224062944248175No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3250.22872183202668656No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3180.22379551564457328No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3160.22238799667825523No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT3120.21957295874561908No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT3030.21323912339718779No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG2950.20760904753191547No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT2890.20338649063296127No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGA2830.19916393373400706No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2630.18508874407082634No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2530.178051149239236No Hit
CCATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG2360.16608723802553238No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2330.1639759595760553No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2130.14990076991287457No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2120.14919701042971553No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA2120.14919701042971553No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2070.14567821301392037No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC2020.14215941559812517No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG2010.14145565611496613No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT2010.14145565611496613No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1990.14004813714864808No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1970.13864061818233No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1950.13723309921601196No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1940.13652933973285292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1920.1351218207665348No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1860.1308992638675806No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG1820.12808422593494448No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG1710.1203428716201951No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC1700.11963911213703604No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1690.118935352653877No Hit
CAAATATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG1670.11752783368755894No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1640.11541655523808184No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATT1620.11400903627176377No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1590.11189775782228664No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1580.11119399833912762No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA1570.11049023885596858No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1550.10908271988965051No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT1550.10908271988965051No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1540.10837896040649148No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA1510.10626768195701437No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA1440.10134136557490112No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1430.10063760609174209No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGTA100.0070845042145.000023
CCGGTGT100.0070845042145.00002145
TACCGAG100.0070845042145.00002145
GAGCGAA1000.0130.51
AACTGCG458.367351E-11112.77778145
GACCCCG203.859157E-4108.75001145
GCAGGAG301.5947162E-596.6666649
GAGCAAA5050.096.1881261
TGCCGTT406.090322E-790.62501145
AGCAGGC2850.086.491238
GCAAAAG5650.084.690263
AAGCAGG8150.074.723937
CAAAAGC6550.073.053444
TAAACTA406.64354E-572.500015
CTGTCCT300.001932313272.5145
CACGAGC651.389435E-766.92307145
TCGTATC451.1903484E-464.44444145
CGAAAGC2050.063.6585354
AGCGAAA2050.063.6585352
GCAGGGT1600.063.4375049
Produced by FastQC (version 0.11.5)