FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID10_Shed_Ind3_D3_rep1_SID10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID10_Shed_Ind3_D3_rep1_SID10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128535
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT15111.1755552962228186No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT7520.5850546543742949No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC5360.41700704088380597No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA4310.33531722877037384No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT4220.3283152448749368No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG3940.30653129497802156No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA3390.2637413933947952No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG3380.26296339518419104No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGA3280.2551834130781499No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3180.24740343097210876No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT3080.23962344886606762No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3000.23339946318123467No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGA2970.23106546854942236No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2740.21317150970552767No Hit
CCATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG2690.2092815186525071No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT2600.20227953475707006No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2520.19605554907223713No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2280.17738359201773835No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2280.17738359201773835No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2160.16804761349048897No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2130.16571361885867664No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2040.1587116349632396No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2030.15793363675263547No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1860.1447076671723655No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC1800.1400396779087408No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1790.1392616796981367No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA1760.13692768506632433No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1680.13070369938149143No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG1660.1291477029602832No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1570.12214571906484614No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1480.11514373516940911No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG1470.114365736958805No Hit
CAAATATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG1470.114365736958805No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1460.11358773874820087No Hit
CCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAAT1450.11280974053759676No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1400.1089197494845762No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1380.10736375306336794No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1380.10736375306336794No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1370.10658575485276384No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1340.10425176022095149No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1330.10347376201034737No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1330.10347376201034737No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1320.10269576379974327No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1310.10191776558913913No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA1310.10191776558913913No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG1300.10113976737853503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGG100.0070837243145.0145
AGATCCG151.2275316E-4145.0145
CTGACGT100.0070837243145.0145
CCGTATC100.0070837243145.0145
TCTCCGC100.0070837243145.0145
ATTGCGT100.0070837243145.0145
AAGTGCG100.0070837243145.0145
CCTGAGT151.2275316E-4145.0145
GAGCGAA900.0120.8333361
AGGTCGT203.8583102E-4108.756
GAGCAAA5700.0104.298251
AGCAGGC2900.0102.500018
AGGGTGT501.9281288E-10101.56
AAGGGTG501.9281288E-10101.55
TGCCCTA301.5941341E-596.666664145
CCCCGAC301.5941341E-596.666664145
GCAAAAG6200.095.887093
AAGCAGG8050.087.360257
CAAAAGC7000.084.928574
CCGAACA353.425865E-582.85714145
Produced by FastQC (version 0.11.5)