FastQCFastQC Report
Fri 7 Dec 2018
000000000-BT95L_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BT95L_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15878222
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGNTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAA1978701.246172272940887No Hit
CCTGTTTGNTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA1729271.0890828960572538No Hit
CCTGTTTGNTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG1607351.01229848027065No Hit
CCTGTTTGNTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1393440.8775793662539798No Hit
CCTGTTTGNTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1025670.6459602340866628No Hit
CCTGTTTGNTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG985340.6205606647897982No Hit
CCTGTTTGNTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG889780.5603776039911773No Hit
CCTGTTTGNTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG650080.4094161172453692No Hit
CCTGTTTGNTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA601240.3786570058032946No Hit
CCTGTTTGNTCCCCATGCTTTCGCGCCTCAGCGTCAGTTATAGACCAGAA571530.35994584280280245No Hit
CCTGTTTGNTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG485390.30569543617667017No Hit
CCTGTTCGNTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG469970.2959840213847621No Hit
CCTGTTTGNTACCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA456270.2873558513037543No Hit
CCTGTTTGNTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGAA401160.25264793501438637No Hit
CCTGTTTGNTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG374600.23592062133908948No Hit
CCTGTTCGNTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG373270.2350829960684515No Hit
CCTGTTCGNTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT369450.23267718514075444No Hit
CCTGTTTGNTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG318960.2008789145283395No Hit
CCTGTTCGNTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG287700.18119157170116404No Hit
CCTGTTTGNTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG266910.16809816615487552No Hit
CCTGTTTGNTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG264210.16639772387613674No Hit
CCTGTTTGNTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA263220.16577422837393255No Hit
CCTGTTTGNTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG190300.11984969097925448No Hit
CCTGTTTGNTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA175000.1102138513997348No Hit
CCTGTTTGNTCCCCACGCTTTCGCACATGAGCGTCAGTATCTTCCCAAGG172630.10872124095506412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCTC383500.06022.4625
CTCCCCA528150.04906.43859
GCTCCCC601650.04367.1998
TTTGATC38850.03645.8315
TTGCTCC734050.03155.32426
TGTTTGC774250.03077.40753
TGCTCCC762750.03034.5547
TGTTTGA57500.02934.8073
TTGATAC10300.02756.35946
TGTTCGA7550.02711.72363
TGTTCGC142700.02478.163
TTTGCTA62450.02243.08425
GTTTGCT1141100.02087.5824
TTGGGTC1850.02023.67717
TGATCCC74050.01912.76897
TTGCTAC81950.01770.25436
TGCTACC84050.01718.60067
CTACCCA136850.01664.21629
GATCCCC99750.01460.61468
TTGATCC100700.01412.75576
Produced by FastQC (version 0.11.5)