FastQCFastQC Report
Fri 7 Dec 2018
000000000-BT95L_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BT95L_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15878222
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGAGAGAAT3681292.3184522801104555No Hit
TGGACACCGAAC2230821.4049557941688937No Hit
CTCCTGAAAGTT1559770.9823329085586535No Hit
GTAAGCACCTAC1386670.8733156646884016No Hit
GCACTACCGAAT1375710.8664131286235952No Hit
CCATTCGCCCAT1350480.8505234402189363No Hit
GTCATTCACGAG1332030.8389037513142215No Hit
TACCCAGAGATC1331460.8385447690553766No Hit
CACATGCCTAAG1330680.838053530174852No Hit
TGTTTGAGCTGT1320250.8314847846314279No Hit
CGTAACCAACCA1316360.829034888163171No Hit
GCGACTTGTGTA1298400.8177237980423753No Hit
ACACGAGCCACA1294900.8155195210143805No Hit
GGTAACAGCTCG1278490.8051846107202684No Hit
TGCGGCATCGAA1241330.7817814866173303No Hit
ACTTATGCGGTT1225550.771843346188257No Hit
TCCAAAGTGTTC1225520.7718244523851601No Hit
GCTAATTACGCT1224350.7710875940643732No Hit
AAGTGGACTCTC1223070.7702814584655637No Hit
ATCTCTGGCATA1222840.7701366059751527No Hit
AAGGAGCGCCTT1217360.7666853379427495No Hit
CACGAGGTCATT1216060.7658666064752087No Hit
CATTTGGACGAC1206490.7598394832872345No Hit
TGGAGCACGTTG1166880.7348933652647003No Hit
ATGGATACGCTC1141410.7188525264352646No Hit
TTCGCCCTTCAG1135080.714865933981777No Hit
AGAACACGTCTC1131830.7128191053129248No Hit
CCTCTACCTACG1131610.7126805507568794No Hit
CCATAATCCGTA1106280.6967278830085635No Hit
GACTGATCATCT1106180.6966649036649066No Hit
CTCGAGAGTACG1093080.6884146096458407No Hit
AGTTTGCAACGC1086420.6842201853582851No Hit
GAGGCCATCAGT1074920.6769775608377311No Hit
ATTCTGTGAGCG1064170.6702072813946045No Hit
GAATCTTCGAGC1060840.6681100692508266No Hit
GAGGCTCATCAT1020720.6428427565756418No Hit
CATCCAAATGCG999610.6295478171296509No Hit
CCTAATGGAACC985660.6207621986895007No Hit
CGAGATACCCAG972500.6124741170642406No Hit
TACCTCTCAGAA970900.6114664475657287No Hit
TGAGGATGATAG963950.6070893831815678No Hit
TGCTATATCTGG959850.6045072300916311No Hit
TTCCGTAGGGAT931230.5864825419370003No Hit
TCCAGGCTTAAT931190.5864573501995375No Hit
CCAGTGTATGCA931080.5863880729215147No Hit
GTGCCGGTGATA926220.5833272768197849No Hit
AAGCCTACACGT903600.5690813492845735No Hit
CGCAAATTCGAC895430.5639359369077974No Hit
GATAGAAAGCCA889480.5601886659602063No Hit
ACACCTGGTGAT886250.5581544331600855No Hit
TACGTCCCGTTC876550.5520454368253573No Hit
CAGCATGTGTTG866770.5458860570157037No Hit
ATTATACCTCGG858430.5406335797547106No Hit
TTCTGGGAACAC838490.5280754986295065No Hit
CTGGAAATCTGC805180.5070970792573627No Hit
GACTTTCCCTCG795530.5010195725944631No Hit
CAACGGGTAGTC789150.49700149046914704No Hit
TAACTCTGATGC787320.49584896848022403No Hit
TAAAGTCACCCT780160.4913396474743835No Hit
TCGCATGAAGTC774990.48808361540731704No Hit
ACACGTAAGCCT770010.48494724409319884No Hit
GACAGGAGATAG755320.47569557850998684No Hit
TGTGAGCACGGT754190.47498391192666284No Hit
CAAGCATGCCTA747830.4709784256700782No Hit
ACCGGCTAGAGT738480.46508985703814953No Hit
CTTACACCAAGT733770.46212352995190525No Hit
ATTCATGGACGC731880.460933220356788No Hit
TCCCAGAACAAC728230.45863447431330784No Hit
ACCCGTATGATG722470.45500686411866514No Hit
CGTGACAATGTC706900.4452009803112716No Hit
CTGGCCTAGGAA687190.43278775167647865No Hit
GGTAGGAACAAT678100.42706292933805806No Hit
ACGCGCAGATAC672530.42355497989636376No Hit
ATGTGCACGACT668040.42072720736616476No Hit
TAGAACTCACCT656830.4136672229422161No Hit
GTTACGCATTAC644100.40564995249468105No Hit
TAAGGCCTATCG643200.4050831384017682No Hit
TCTGTTGCTCTC642430.4045981974556094No Hit
GACTCGAATCGT632610.39841362590849283No Hit
AATCCGTACAGC627950.39547878849407697No Hit
TATCGTTGACCA623610.3927454849793636No Hit
AGTTCCCGAGTA620070.3905160162139061No Hit
TGAGTCACTGGT605800.3815288638740534No Hit
TGATTTGGACCT604150.38048970470371307No Hit
GACGGAACCCAT601980.37912305294635634No Hit
CTAGCGAACATC598190.37673613582175636No Hit
GACTTGGTATTC596330.37556472002973634No Hit
ATGTCGAGAGAA594200.3742232600098424No Hit
AGATAGGACAGG566810.3569732177821925No Hit
AGCCTAAGCACG552670.3480679385890939No Hit
TTGCAGACAGGC540590.34046003387532936No Hit
TTCCTAGGTGAG537760.3386777184498365No Hit
CCTTGGCTATCC532570.33540909051403867No Hit
GACACATTTCTG532460.33533981323601597No Hit
GCTTCGGTAGAT530450.3340739284285104No Hit
TTGATGCTATGC530440.33406763049414473No Hit
AAGAGATGTCGA529320.33336226184518647No Hit
CAAGTTTACGGC528170.33263799939313105No Hit
TACAGATGGCTC523780.32987320620658916No Hit
GTCGTCCTAAAT523510.32970316197871524No Hit
CGGAGCTATGGT506470.3189714818195639No Hit
GTCATATCGTAC505810.3185558181514278No Hit
CTACCGGATCAA499260.3144306711418948No Hit
TCAACAGCATCG498970.31424803104528953No Hit
TTACGAGACGGC497230.3131521904656579No Hit
GTATTACGATCC495840.31227677758882577No Hit
CGGGTTTGACGA474860.299063711289589No Hit
TCTACGGAGAGC459770.289560128331749No Hit
GTGGGATGTTTC451840.28456586637974957No Hit
ATACTTCGCAGG444730.2800880350457375No Hit
CAGCTAGAACGC440520.27743660467777814No Hit
ACGATGCGACCA439960.27708392035329904No Hit
CCATAGGGTTCA438990.2764730207198262No Hit
ATGATGACCCGT437820.2757361623990394No Hit
CGTCCGAAATAC435900.2745269590008252No Hit
CTAACCTCCGCT431650.271850336895403No Hit
CAGCGGTGACAT424420.26729693034900254No Hit
ACTCACGGTATG421540.2654831252516812No Hit
AATGGAGCATGA400540.25225746308371305No Hit
AATCAGTCTCGT395690.24920296491634897No Hit
TAGGATTGCTCG387990.24435355545476065No Hit
AAGGTGTAAGGT386520.24342775910300285No Hit
TGGCATACGGCA382280.2407574349319464No Hit
TTATCACGTGCA372010.23428945633837342No Hit
TGACCTCCAAGA368880.23231820288190955No Hit
ATACCTTCGGTA360590.22709721529274499No Hit
TAAACCGCGTGT355610.22396084397862684No Hit
TCCTCTGTCGAC353460.22260678809000153No Hit
CCAAGTCTTACA348870.21971603621614563No Hit
ATGGTTGTTGGC343660.21643481241161636No Hit
TGGCAAGACTCT339240.21365112542197734No Hit
TCGTCGATAATC331650.2088709932384117No Hit
TACGATGACCAC330630.2082286039331104No Hit
ATCCCGAATTTG319350.20112453396860178No Hit
AGCCGGCACATA316700.1994555813616915No Hit
CGCACATGTTAT314330.19796297091702078No Hit
GCACACACGTTA313610.19750951964269048No Hit
ACCACATACATC312320.19669708610951528No Hit
GTCCGAAACACT295980.1864062613559629No Hit
CTAGATTTGCCA294940.18575127618193019No Hit
GGTCAGCTTAAC293640.1849325447143893No Hit
CCATACATAGCT291370.18350291361337562No Hit
AGCTTGACAGCT288260.18154425602564317No Hit
GCCTGAATTTAC285580.17985640961563581No Hit
CTCTACCTCTAC282460.17789145409353768No Hit
AATGTCCGTGAC281670.17739391727864742No Hit
TAGGAACTGGCC279660.17612803247114192No Hit
CAGGAAGGTTAA265570.16725424294987185No Hit
AATCTTGCTGCA264890.1668259834130043No Hit
TAGCGGATCACG264190.16638512800740538No Hit
TAATCCACAGCG261540.1647161754004951No Hit
TTATGCAGTCGT254780.1604587717692825No Hit
GTTGGTCAATCT248580.15655405246254903No Hit
ATTGGGCTAGGC243980.1536570026543274No Hit
TAAGGTAAGGTG230630.14524926027611906No Hit
CAGGCGTATTGG213650.13455536772316196No Hit
ATTAGTTCGCGT201100.12665146009420955No Hit
CGGACTACAACT199620.12571936580808607No Hit
CCATGCGATAAC199370.12556191744894357No Hit
TGGCACCGATTA193120.12162570847038164No Hit
TACTAATCTGCG186460.11743128418282602No Hit
TACTTCGCTCGC181890.11455312817770151No Hit
GGAGACAAGGGA180900.1139296326754973No Hit
TGCAATGTTGCT179430.11300383632373952No Hit
TCGGAGTGTTGT178140.11219140279056435No Hit
AGAGCCTACGTT177230.11161829076328572No Hit
CGACTGTCTTAA170920.10764429417852955No Hit
GAGGAATAGCAG168000.10580529734374541No Hit
TAGCTCGTAACT165710.10436307037400032No Hit
CGCCAAATAACC165190.10403557778698397No Hit
ACTATTGTCACG163900.10322314425380878No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGTC550.0091607286.04
AGCCAGG550.0091607286.05
GGGGTTG550.0091607286.02
GAGCGTC650.00126197496.04
CGTGTAG600.00339657466.04
AAGGGGC550.0091607286.01
GAACCGT550.0091607286.06
CAGTAGG650.00126197496.03
AAGGCGT1701.8189894E-126.01
CACGCGG1552.7284841E-115.99999951
CGAGGTC127200.05.99764163
ACGAGGT127100.05.99763972
CGATGCG46950.05.99361042
ACACGAG134250.05.9932961
GTGCCGG101250.05.99111131
GCCGGTG100700.05.99106263
TGCCGGT100400.05.9910362
GTGGACT127750.05.99060633
ACGGGTA83600.05.98923443
CAACGGG82750.05.9891241
Produced by FastQC (version 0.11.5)