FastQCFastQC Report
Wed 12 Jun 2019
000000000-BT4RC_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BT4RC_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862531
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT49140.5697186535904217No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA37490.4346510444262293No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAT26330.30526439049726906No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATA21950.25448360696601047No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC9280.10759033588357983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAAGG1109.921132E-846.1576231
TAATATA26350.013.4881851
CGCCGCA1357.9830774E-510.74070690-94
TATTATT102400.09.1332723
ATTATTA111800.08.2394841
TTATTAT122400.07.7001392
AATATAT51100.07.51954562
AATAATA97000.06.42781452
TAATAAT101250.06.3041381
GGTGGGC3350.00271391085.626847375-79
ATAATAA111550.05.5894046
TATTATA47602.0008883E-115.33333871
TAAGATC8056.2536856E-95.223585635-39
Produced by FastQC (version 0.11.5)