FastQCFastQC Report
Wed 12 Jun 2019
000000000-BT4RC_l01_n02_ISO_525_Jun10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BT4RC_l01_n02_ISO_525_Jun10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9313623
Sequences flagged as poor quality0
Sequence length151
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT4474904.804682345420252No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA3500983.7589883120671725No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAT1971202.1164696058665893No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATA1695551.8205052963814403No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAA426030.45742671783042965No Hit
AATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAA415220.44582006379257566No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAAGAT339050.36403663751474585No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAGAT319830.34340019990072607No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTAAGATCGG250090.2685206390681693No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAGATCGG234380.25165287450436846No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAG222310.23869336347412817No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG187060.20084557856808247Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA176610.1896254550994817Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATATATATATATATATATATATATATATAGAT172770.18550246236078055No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTAAGATCGGAAG158590.17027745271630598No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT152080.1632876915889767No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAGATCGGAAG151350.1625038934902132No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG143800.15439748849615237Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT143340.15390358832432877No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG142530.153033894543509Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC141150.15155219402803827Illumina Single End PCR Primer 1 (100% over 40bp)
ATATATATATATATATATATATATATATATATATATATAGATCGGAAGAG139630.1499201760689691No Hit
TATATATATATATATATATATATATATATATATATATATATATATATAAG136260.14630182046234855No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTC135510.14549654844307097No Hit
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCG133060.1428659931800976No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA123160.13223640252563368Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG119610.12842478163438653Illumina Single End PCR Primer 1 (100% over 28bp)
ATTATTATTATTATTATTATTATTATTATTATTATTAAGATCGGAAGAGC115660.12418368233285801No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG115010.12348577991615078No Hit
ATATATATATATATATATATATATATATATATATATATATAGATCGGAAG112570.12086596161343442No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG112380.12066195936855079No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT111620.11984595038901619No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAGATCGGAAGAGC111000.11918025885308005No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC108200.11617390998111046No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG107960.11591622293494165Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATATATATATATATATATATATATAAGATCGGAAG105210.11296355886425723No Hit
ATATATATATATATATATATATATATATATATATATATATATATAGATCG96960.10410556665220397No Hit
ATATATATATATATATATATATATATATATATATATATATATAGATCGGA96500.10361166648038042No Hit
TATATATATATATATATATATATATATATATATATATATATATATAAGAT96430.10353650775858116No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT94920.10191522675976901No Hit
TATATATATATATATATATATATATATATATATATATATATATAAGATCG93890.10080931985329447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATA911050.09.963231
TTATTAT6780750.09.2796152
TATTATT6787650.09.2114363
CGCACAA8004.4235436E-49.0625145
ATTATTA7087100.08.8181341
TAATAAG117600.08.6309526
TAATAGG53950.07.25671961
ATAATAA6046400.07.2543173
TAATAAT6185300.07.2473041
ATATTTG273700.07.12550266
AATAATA6406100.06.9239482
TATTATA829400.06.57342671
CGAACGC2450.0039231316.510204120-124
TATAAGG58300.06.46655277
TATTAAG408350.06.143014445-49
AATATAT1467800.06.0408432
ATTAAGA415000.05.985181345-49
TAATAGA473700.05.78224645-49
TATATGG111600.05.52195366
TAAGATC533150.05.431210545-49
Produced by FastQC (version 0.11.5)