FastQCFastQC Report
Wed 12 Jun 2019
000000000-BT4RC_l01_n02_ISO_349_Jun10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BT4RC_l01_n02_ISO_349_Jun10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8970734
Sequences flagged as poor quality0
Sequence length151
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGC401730.4478228871795775No Hit
AAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGT394170.439395483134379No Hit
AGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACT375300.4183604151009271No Hit
TACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATT370210.4126864089382207No Hit
ATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAA367680.4098661268966397No Hit
TTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTG366780.4088628645103065No Hit
AATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAA364380.4061874981467514No Hit
AATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATT345160.38476227251861445No Hit
TAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGA340350.3794003924316561No Hit
AATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGA331830.36990284184103556No Hit
ACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTT330930.36889957945470236No Hit
TATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGG330470.3683868009016876No Hit
TAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATAT326620.36409506736015135No Hit
AACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAA326030.36343737312911073No Hit
AGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCT324210.3614085536367481No Hit
CAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTG322060.3590118712693967No Hit
CAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTG321630.3585325347959264No Hit
TTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAAT318040.35453063261044193No Hit
ATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTT317840.354307685413479No Hit
TGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAAT317360.35377261214076794No Hit
AAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATA312760.3486448266106207No Hit
AAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATA309360.344854724262251No Hit
TTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTT307950.3432829465236624No Hit
CAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCAC304880.3398607070502815No Hit
ATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGA304400.33932563377757047No Hit
TATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGC304240.33914727602000017No Hit
TTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAA303850.3387125289859224No Hit
GTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAA303480.33830007667154105No Hit
ATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAA302110.336772888372345No Hit
AAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTA302020.33667256213371166No Hit
TCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGT296540.33056380893692755No Hit
ATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAA296230.33021824078163503No Hit
TAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAAT294840.32866875776274274No Hit
TGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAAT294410.3281894212892724No Hit
ATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTG294200.32795532673246136No Hit
AATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACA293620.3273087798612689No Hit
ATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCA292320.32585962308100985No Hit
GTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATA287800.3208210164296478No Hit
AAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTG285580.3183463025433593No Hit
GGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCA284620.3172761559979373No Hit
GAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATC284330.31695288256234105No Hit
AGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGT283540.3160722411343375No Hit
GTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTG282250.3146342317139267No Hit
TATATATATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGT281400.3136867061268342No Hit
ACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAG279700.3117916549526493No Hit
ATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAA279390.31144608679735686No Hit
TACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTC278650.310621182168594No Hit
TATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTT275420.3070205849376428No Hit
TTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTT274460.30595043839222075No Hit
TTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATT274440.30592814367252447No Hit
AATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATAT269780.3007334739832883No Hit
CAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATA268190.2989610437674331No Hit
AATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGC267930.2986712124113813No Hit
CTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTG266770.29737811866899627No Hit
TATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAA265440.2958955198091929No Hit
TAATATATATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTG265010.2954161833357226No Hit
GGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACC264550.2949034047827079No Hit
GTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGG263370.2935880163206266No Hit
CACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAA263190.29338736384336No Hit
TTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTT260830.2907565869191975No Hit
CAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATAT260320.29018807156694204No Hit
ATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATT260280.2901434821275494No Hit
GGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACC258240.2878694207185276No Hit
ATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCA256320.28572912762768354No Hit
TAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTT256170.2855619172299614No Hit
CATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCA255170.28444718124514673No Hit
ATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATT253970.28310949806336916No Hit
TGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCA251760.28064593153692885No Hit
TGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACG251320.2801554477036104No Hit
TTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCAC250930.27972070066953275No Hit
ACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCA249920.27859481732486996No Hit
TATATATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACT249690.27833842804836256No Hit
CGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACA248040.2764991136734185No Hit
TGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATT247540.27594174568101115No Hit
TGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCC247170.27552929336662974No Hit
ATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGC247040.27538437768860385No Hit
AAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGA247020.2753620829689076No Hit
ATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAG246480.27476012553710766No Hit
ACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTG244460.27250835884778213No Hit
ATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACA241070.2687294038592606No Hit
AATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGAT240280.267848762431257No Hit
AATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTA239790.2673025417986979No Hit
TTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCT239270.26672287908659426No Hit
ATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAA236640.2637911234465318No Hit
TATTATATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTT235920.2629885135374653No Hit
TTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGT235360.2623642613859691No Hit
GGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAA233130.2598784001398325No Hit
TATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTG231500.25806138048458466No Hit
TTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAAT229760.2561217398710072No Hit
GAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTA229640.2559879715528295No Hit
CAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTT229200.25549748771951103No Hit
CAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTG227990.2541486571778853No Hit
AAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAA227950.2541040677384927No Hit
CACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGT227040.2530896579923114No Hit
TACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAAC226610.25261032151884116No Hit
ACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGA226400.2523762269620301No Hit
AATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAAT226340.2523093428029412No Hit
ACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACT224130.2498457762765009No Hit
TGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATG223340.24896513484849733No Hit
ATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTA221970.24743794654930132No Hit
CTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGT221840.2472930308712754No Hit
CACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGT220370.2456543689735979No Hit
ACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTG219490.24467340130696102No Hit
CAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAG218380.24343604436381683No Hit
CACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCA217840.24283408693201694No Hit
CCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGC217350.24228786629945778No Hit
CTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAA216040.24082756215935064No Hit
TGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCA215930.24070494120102098No Hit
TGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGT215780.24053773080329882No Hit
CACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTG215670.24041510984496922No Hit
TGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCAC215310.24001380489043594No Hit
AAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAAT214310.23889906890562132No Hit
GTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCA214090.23865382698896212No Hit
TTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATA212150.23649123917842174No Hit
ATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTA210690.2348637246405924No Hit
ACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAA210540.2346965142428702No Hit
TTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATAT209760.2338270201747148No Hit
ATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATT207180.2309510013338931No Hit
CTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAAT206090.22973593911044515No Hit
TTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGC205990.22962446551196367No Hit
GCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAA205980.22961331815211555No Hit
CAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATAC205930.2295575813528748No Hit
ATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCA203960.22736155146279No Hit
AAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAA203940.22733925674309372No Hit
GTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTG203370.22670385723174935No Hit
CTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTC202600.22584551052344215No Hit
AATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATT201940.22510978477346447No Hit
TTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTC200820.2238612804704721No Hit
GTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCA200410.22340423871669812No Hit
TATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTAC200350.22333735455760925No Hit
AATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTAT197540.22020494644028016No Hit
CCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTAC196700.21926856821303584No Hit
TAATATATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTT195860.21833218998579157No Hit
GTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTA195680.21813153750852493No Hit
GATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTC195050.21742925383809175No Hit
GAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAA194380.21668238072826596No Hit
TTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTAT194340.21663779128887334No Hit
TGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCAC194260.2165486124100882No Hit
GTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCA193770.21600239177752903No Hit
TCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATT193060.21521092922831062No Hit
GCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATA191640.21362800412987387No Hit
TCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAAT191370.21332702541397391No Hit
TTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAA191190.21312637293670728No Hit
ATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATT188310.2099159333004412No Hit
TTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTAT187540.20905758659213394No Hit
GCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATT186200.20756384037248235No Hit
GCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCG186100.20745236677400086No Hit
CCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATA185040.2062707466300974No Hit
CCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGC184240.2053789578422457No Hit
TATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTT180330.20102034014162054No Hit
TGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCAT180280.20096460334237978No Hit
GCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGG179480.20007281455452808No Hit
ACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAA178270.19872398401290242No Hit
CGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGC177970.19838956321745804No Hit
TGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTG176480.19672860660008423No Hit
TTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCC176130.19633844900539912No Hit
AGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAA174020.19398635607744025No Hit
TGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCAC171260.1909096847593519No Hit
GCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAAT165140.18408750053228645No Hit
TGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAAT164260.18310653286564957No Hit
GCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGG162480.18112230281267955No Hit
GAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAG154300.17200376245689594No Hit
TCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTA153730.1713683629455516No Hit
GCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACT151590.1689828279380483No Hit
TTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGT140910.15707744762022818No Hit
TCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTT136590.15226178816582903No Hit
TTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAG134320.14973133748029982No Hit
TATATATATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGA110830.1235461891970044No Hit
TATATATATAATTATAGATAGATGATAGATAGACAGATAGATAGATGCTT109400.12195211673871949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG3252.206152E-517.846153145
TCTCGGG5354.517733E-714.906543145
TAATATA1017800.013.6338191
TATATCG12601.0351869E-89.7817466
GGTAGGG12408.4299245E-89.354839145
AGTAGTC13553.385685E-89.0959416
GACGGAA12851.4577927E-79.027238145
CACCACG25000.08.41145
AGGAGTA15252.2786298E-78.0819673
GCCCCAC13854.1030307E-67.8519855145
GTAGTCT16306.5915447E-77.561357
GCGTGTC2159.886642E-47.4186044135-139
AATCTGG19653.0120646E-87.379135145
CAACTGG48500.07.3247423145
AATATAT2074600.07.28284932
TCTGCGG2200.00126229337.25140-144
TTTCCGG44150.07.2253685145
TGCACCC35800.07.087989145
GAGTAGT17652.3170396E-66.98300275
ATTTGCC25401.5024852E-96.850394145
Produced by FastQC (version 0.11.5)