FastQCFastQC Report
Wed 12 Jun 2019
000000000-BT4RC_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BT4RC_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862531
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT67540.7830443195664851No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA51160.5931381017030113No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATA30930.35859580699128496No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAT25950.3008587517434156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTCG100.007090694145.06
TAATATA28150.011.07461
AGACCGA2250.00160086419.666667145
ACGTGAT7600.00279480668.5855266
TATTATT98300.08.48168859
CGGTTCA5152.0154403E-98.446603145
TGATTAC8050.00469781748.1055919
GGTTCAG5500.07.909091140-144
ACCGATC2107.689341E-47.595238140-144
ATTATTA110200.07.5657897
TTATTAT121100.07.0045422
TAATAAT122150.06.88497731
AATAATA122300.06.8765332
CCGAGAC2500.00484192846.38135-139
ATAATAA138800.06.1113113
AGCGGTT5251.4166653E-76.07619140-144
AATATAT54250.05.6129032
CAGATCG3150.0084909155.5238094125-129
TATTATA48052.5465852E-115.2809571
GGAATGC4454.88854E-45.2134833130-134
Produced by FastQC (version 0.11.5)