FastQCFastQC Report
Wed 12 Jun 2019
000000000-BT4RC_l01_n01_ISO_525_Jun10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BT4RC_l01_n01_ISO_525_Jun10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9313623
Sequences flagged as poor quality0
Sequence length151
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT4885315.2453379313291935No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA3737364.012788578622948No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATA2041612.1920685430363673No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAT1790141.9220662034527274No Hit
AATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAA467580.5020387876984069No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAA382570.4107638885533589No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAGAT367620.3947121329690927No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTAAGAT320660.3442913676020599No Hit
TAATAATAATAATAATAATAATAATAATAATAATAATAATAATAGATCGG272430.2925070082823838No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTATTAAGATCGG236210.25361773823140576No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAG222600.23900473532158217No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA221560.23788809145485057No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG212960.22865430563380112No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC212380.22803156193889318No Hit
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCA197450.21200128027514106No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC192860.2070730155171623Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATATATATATATATATATATATATATATAGAT189070.20300370757974634No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG187900.20174748322967337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATATATATATATATATAGATCGGAAGAG187880.2017260093091593No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC178000.19111789257520945Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA175310.18822965026606725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAATAATAATAATAATAATAATAATAATAATAATAATAATAGATCGGAAG172070.18475087514278815No Hit
ATATATATATATATATATATATATATATATATATATATATAGATCGGAAG161520.17342338207161703No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTATTAAGATCGGAAG151080.1622139955632733No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA150640.16174156931196376No Hit
ATATATATATATATATATATATATATATATATATATATATATAGATCGGA150240.16131209090168241No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG145300.1560080325347075No Hit
TATATATATATATATATATATATATATATATATATATATATATATATAAG145050.15573960852828164No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT144190.1548162299461767Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA143660.15424717105255387No Hit
ATATATATATATATATATATATATATATATATATATATATATATAGATCG136100.146130029098236No Hit
TATATATATATATATATATATATATATATATATATATATAAGATCGGAAG136000.14602265949566565No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA135510.14549654844307097TruSeq Adapter, Index 12 (100% over 39bp)
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG132100.1418352449954223No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC128510.137980676263147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATAATAATAATAATAATAATAATAATAATAATAATAGATCGGAAGAGC126270.13557559716557133No Hit
TATATATATATATATATATATATATATATATATATATATATATAAGATCG121180.1301104843947409No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC115590.12410852361105877No Hit
TATATATATATATATATATATATATATATATATATATATATAAGATCGGA113920.12231545124813405No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG113630.12200407940068006Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATATATATATATATATATATATAAGAT109840.11793477146326409No Hit
ATTATTATTATTATTATTATTATTATTATTATTATTAAGATCGGAAGAGC109220.11726907992732796No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG103080.11067658632950893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAG114000.09.921056
GAATCGG5900.0034068429.8305051
TTATTAT6076150.09.3498332
TATTATT6031150.09.3378513
TAATATA888950.09.2811721
ATTATTA6324750.09.0579841
ATAATAA6841100.07.4300533
TAATAAT7041000.07.36429261
AATAATA7258300.07.081892
TCGCGCT3253.885552E-56.6923056135-139
ATATTTG253800.06.62726356
TATTATA804900.06.57534931
TAATAGG47700.06.2316547
TATTAAG371350.06.075667445-49
TAATAGA516250.06.06401845-49
ATTAAGA372950.06.041826745-49
TATATGG87050.05.66340976
AATATAT1420100.05.6464322
ATAATAG521500.05.60536745-49
TTAAGAT365650.05.412961550-54
Produced by FastQC (version 0.11.5)