FastQCFastQC Report
Wed 12 Jun 2019
000000000-BT4RC_l01_n01_ISO_349_Jun10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BT4RC_l01_n01_ISO_349_Jun10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8970734
Sequences flagged as poor quality0
Sequence length151
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGC469920.5238367339840865No Hit
AAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGT410620.4577328900845795No Hit
TTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTG409680.4566850382588537No Hit
TACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATT407910.45471195556573185No Hit
AATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAA396730.4422492072555044No Hit
AGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACT393110.4382138629904755No Hit
ATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAA392420.4374446951609534No Hit
ACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTT377520.4208351289872156No Hit
TGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAAT373820.41671060584340147No Hit
GTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAA362280.4038465525786407No Hit
AGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCT355700.3965115897985605No Hit
AACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAA353290.39382507607515727No Hit
TTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAAT349850.389990384287395No Hit
TAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGA348760.38877532206394705No Hit
CAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTG348640.38864155374576925No Hit
AATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATT347570.3874487862420177No Hit
ATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGA347110.38693600768900294No Hit
AATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGA345130.38472883043906997No Hit
TTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAA344100.38358065237471095No Hit
ATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCA339540.37849745628395626No Hit
GAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATC337680.37642404735220103No Hit
AAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATA336460.37506406945072723No Hit
TATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGG335220.3736817968295571No Hit
TCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGT332350.37048250455313914No Hit
GGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCA332010.37010349431830214No Hit
CAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTG330060.36792975914791365No Hit
AAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTA325270.3625901737806516No Hit
GTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATA325100.3624006686632331No Hit
ATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAA325090.362389521303385No Hit
TATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGC324260.3614642904359889No Hit
TAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATAT323940.36110757492084816No Hit
AAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATA323000.36005972309512246No Hit
ATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTT322580.3595915339815003No Hit
TTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTT322510.35951350246256325No Hit
TGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAAT318550.35509914796269737No Hit
GTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGG315240.35140937185296095No Hit
AGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGT315100.35125330881508693No Hit
GTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTG314890.35101921425827587No Hit
CAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCAC312470.34832155317502445No Hit
GGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACC311490.34722911190990613No Hit
TAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAAT309960.3455235658531398No Hit
ACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAG309720.3452560292167843No Hit
GGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACC304990.3399833280086111No Hit
TTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATT299560.33393031161106773No Hit
AATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACA297800.331968376277794No Hit
ATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAA295380.3292707151945426No Hit
AAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTG294730.32854613680441314No Hit
CTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTG294190.3279441793726132No Hit
CAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATA293850.3275651691377762No Hit
ATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCA292920.3265284646718986No Hit
TACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTC289720.32296130952049185No Hit
TGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACG289280.3224708256871734No Hit
AATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGC288020.321066258346307No Hit
CGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACA285860.3186584286191074No Hit
CAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATAT280990.3132296643730602No Hit
TTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCAC280880.3131070434147306No Hit
TGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATT280570.3127614752594381No Hit
AATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATAT280360.312527380702627No Hit
CACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAA279510.31157985511553454No Hit
ATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAA278670.3106434768882903No Hit
ATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATT278560.3105208559299607No Hit
CATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCA274760.3062848591876651No Hit
TGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCC273770.30518127056269867No Hit
ACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCA272570.3038435873809211No Hit
TTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTT272460.3037209664225915No Hit
TGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCA272450.30370981906274336No Hit
TAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAAATATTT271930.3031301563506397No Hit
ACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTG271910.30310786163094344No Hit
ACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTG269610.30054396886586987No Hit
ATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTG268720.2995518538393848No Hit
GGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAA267770.2984928546538109No Hit
TAATATATATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTG266810.2974227081083889No Hit
ATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATT261770.29180443874492323No Hit
ACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGA259510.28928513541924217No Hit
TGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATG258630.2883041677526053No Hit
TATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGAAA257930.28752385256323504No Hit
GAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTA255720.2850602860367948No Hit
ACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACT255390.28469242316180593No Hit
TATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTT254920.28416849724894305No Hit
GTGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCA253770.28288655086640624No Hit
CACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGT253170.2822177092755175No Hit
CAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTT252230.2811698574497917No Hit
AATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTA251910.280813141934651No Hit
TTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTT250100.2787954698021366No Hit
ATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGC250020.2787062909233514No Hit
ACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAA249150.2777364706165627No Hit
TATATATATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGT249140.27772532325671456No Hit
ATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAA249050.27762499701808124No Hit
AAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGA248530.2770453343059776No Hit
AATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGAT248180.2766551767112925No Hit
TTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAAT247300.27567420904465567No Hit
CAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTG247000.2753397882492113No Hit
TACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAAC245260.2734001476356338No Hit
TTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCT245030.27314375835912647No Hit
ATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAG244270.27229655901066735No Hit
ATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACA243440.2713713281432712No Hit
TATATATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACT243060.2709477284690417No Hit
TGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCA242800.2706578971129899No Hit
CAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAG242530.2703569183970899No Hit
CTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAA241190.2688631721774383No Hit
TTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGC240670.26828350946533475No Hit
CACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGCA240310.26788220451080147No Hit
GTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCA240050.26759237315474965No Hit
GTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTG238910.266321574132061No Hit
TGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGT237540.26479438583286496No Hit
AATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAAT237160.2643707861586354No Hit
TGGTGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCAC235920.2629885135374653No Hit
TTTATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGT235180.2621636089087025No Hit
TATTTATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTTG234760.2616954197950803No Hit
CTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGT231250.25778269648838104No Hit
CACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTG230940.25743712833308846No Hit
TTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATAT230900.2573925388936959No Hit
AAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAA229770.25613288723085537No Hit
CACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGT229420.25574272963617023No Hit
AAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAA229070.2553525720414851No Hit
GTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCA228800.2550515933255852No Hit
CCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAAAGC228750.25499585652634443No Hit
GTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTA228740.2549847091664963No Hit
GCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCCAA228090.25426013077636683No Hit
ATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTA227850.2539925941400113No Hit
GCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATA227520.25362473126502244No Hit
CCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTAC227170.2532345736703373No Hit
GCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATT226350.25232049016278935No Hit
CTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAAT225870.25178541689007833No Hit
TATTATATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTT225540.2514175540150895No Hit
TTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATA224620.25039199690906006No Hit
ATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCA222110.24759400958717534No Hit
CTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTC220730.24605567392813119No Hit
CAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATAC220010.24525306401906466No Hit
TTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTAT218860.24397111763652785No Hit
TGGTGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCAC218750.24384849667819825No Hit
ATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATT216030.24081641479950247No Hit
GCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGG215190.2398800365722582No Hit
GATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTC214300.2388879215457732No Hit
GCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCG213460.23795154331852889No Hit
GAATCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAA212710.2371154913299179No Hit
AAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAAT212100.23643550237918098No Hit
TTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTC211060.23527617695497383No Hit
AATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTAT208170.23205458995885958No Hit
ACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAA207050.2308060856558672No Hit
TCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTATATT206380.2300592125460414No Hit
TCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAAT206190.2298474127089266No Hit
CCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATA202800.22606845772040504No Hit
AATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATT202410.22563371068632732No Hit
CGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGC202200.22539961612951628No Hit
TTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAA201290.22438520638333498No Hit
TGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTG199990.22293604960307595No Hit
CCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTTCCGTGC198690.22148689282281697No Hit
TGCAGATTCCAAAGCAACTGTGAAAATATTTGCACGGAAATTTGCACCAT196730.21930201029258029No Hit
TTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTTAT196320.2188449685388063No Hit
TATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTAC194910.21727319080021767No Hit
TGCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCAC194030.2162922231335808No Hit
TTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAGATTCC192680.21478732955408109No Hit
AGCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAA191440.21340505693291095No Hit
TGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAAT190870.2127696574215666No Hit
GCAACTGTGAAAATATTTGCACGGAAATTTGCACCATACTCAATATAAAT190310.21214540527007045No Hit
ATATTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTA189610.21136509008070017No Hit
TATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATTT188690.21033953297467076No Hit
GCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGG188090.209670691383782No Hit
TAATATATATTGAGTATGGTGCAAATTTCCGTGCAAATATTTTCACAGTT186660.20807661892549706No Hit
GCAAATTTCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACT180740.20147738189539452No Hit
ATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTACAATTTATT176520.1967731960394768No Hit
TCCGTGCAAATATTTTCACAGTTGCTTTGGAATCTGCACCACTGTAATTA176250.19647221732357686No Hit
GAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGTGGTGCAG174950.19502306054331786No Hit
TTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAGT153700.17133492086600718No Hit
TCTGCACCACTGTAATTACAATTTATTTATATTGAGTATGGTGCAAATTT150540.16781235515399298No Hit
TTTGCACGGAAATTTGCACCATACTCAATATAAATAAATTGTAATTACAG141000.15717777385886147No Hit
TATATATATAAATTGTAATTACAGTGGTGCAGATTCCAAAGCAACTGTGA109120.1216399906629714No Hit
TATATATATAATTATAGATAGATGATAGATAGACAGATAGATAGATGCTT100760.11232079782992116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG9550.019.7382186
TAATATA1016550.013.7219031
TTTCCGG6806.683409E-611.727941145
GCCCGGG4700.007138230410.797873145
AGTAGTC17450.010.3868193
GTAGTCT17650.09.8583564
CAGTGGG18951.8189894E-129.182058145
TTATTGA65250.08.6666678
GAGTAGT20801.6370905E-108.0168282
GCGGGGT2107.693449E-47.595238135-139
AGGAGTA17302.1084088E-77.54335264
CCCGATT1950.0031306427.435897135-139
TAGTCTG22458.221832E-107.4276175
CCGATTT2159.886642E-47.4186044135-139
CTGGCAC25502.910383E-117.39215669
AATATAT2060050.07.27445942
GGAGTAG23452.050001E-97.1108741
ATTATTG90350.06.90094047
ATTTGCC15351.336143E-46.6123776145
TCTGGCA26654.2327883E-96.5290813
Produced by FastQC (version 0.11.5)