FastQCFastQC Report
Mon 22 Oct 2018
000000000-BRWHK_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BRWHK_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14428038
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGTTGAAAGTTTTGAAGATTAATTCAAAATTTGACTATCAATAAAAA196921213.648508549811138No Hit
CGTTGTTGAAAGTTTTGATTTATTTGTTTTTTATACTCAGAAGTTCCACT12924638.957995536191406No Hit
CGTTGTTGAAAGTTTTGATTTATTTTGTTTTCGGGCTTTCGCCCCTCAGA7993445.540212744102837No Hit
CGTTGTTGAAAGTTTTTAATATTTTAAAATTTCCAGTTACGAAAATTCTT7661165.309911160477953No Hit
CGTTGTTGAAAGTTTTGATTTATTTATGGTTTTACTCAGAAGTTACATAT5920004.1031219906684475No Hit
CGTTGTTGAAAGTTTTGACTTATTAAATAAGACGCTCAGATAATCAACTA5039653.4929558682892297No Hit
CGTTGTTGAAAGTTGTAATTAATTACATTGTTTTTACTGACGCTGATGGT1840491.2756342892914476No Hit
CGTTGTTGAAAGTTTTAATTATTGTTTAACTAAAAACTCAGACTGCAAAC1573481.0905710118035454No Hit
CGTTGTTGAAAGTTATATTAGTTTATAACGATTTTACATTCAATGACTTG1253140.8685449816530841No Hit
CGTTGTTGAAAGTTTTGAAGATTTTTTGAATTTAATCAACAAATTGACAA1228590.8515295011005654No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAGTTTACTCAGACTGCAAAGT1187900.8233274683640284No Hit
CGTTGTTGAAAGTTTTGATTTATTTGTTTGTTTTACTCAGAAGTTCCACT1171110.8116904044749536No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACAGAA1038280.7196266048093303No Hit
CGTTGTTGAAAGTTTTGAAGATTTATTTGAAATTAATCAACAAATTGACA1028030.7125223817680546No Hit
CGTTGTTGAAAGTTTTGATTGATTTAATAATCTTACTCAGACGCATTGAT779900.5405447365747165No Hit
CGTTGTTGAAAGTTTTGATTCATTTGTGTTTTCTGCTCAGAGTTTCGGTA765950.5308760622892731No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAAAATTTAATACATTCATAGACTT676130.46862227559977315No Hit
CGTTGTTGAAAGTTGTAATTATTAATTTGTTACTGACGCTGATTGCAATT635030.44013607394158516No Hit
CGTTGTTGAAAGTTGTAACTATTAAGTTTTTTCAGACGCTGATTTCAACT622070.4311535636376893No Hit
CGTTGTTGAAAGTTATTTTAGTTTATAAGTTTTTACATTCAATGACTTGT539330.37380688905865095No Hit
CGTTGTTGAAAGTTGTAACTATTAAGTTTTTTCAGACGCTGATTGCAACT445910.3090579606180688No Hit
CGTTGTTGAAAGTTTTGAAGATTTTAATTTTTGTTAAAAATTTTCATGAC442950.30700639962273457No Hit
CGTTGTTGAAAGTTTTAACGATTGTTTAACTAAAAACTCAGACTGCAAAC432050.29945166487640246No Hit
CGTTGTTGAAAGTTTTGTATACTATTAAAGTAACTAAATCAGACTACAAG415430.28793242712557315No Hit
CGTTGGGAGGAGATTTAAATAATCTGATTGTGTATATGTATTATCCTAGA398580.2762537775406469No Hit
CGTTGTTGAAAGTTTTGATTCATTTTGTTTTCGGGCTTTCGCCCCTCAGA388810.26948224006618227No Hit
CGTTGGGAGGAGATTTAGTAATCTGTGTTTGTGTATGTGTATTAGCCTAG328750.2278549585189615No Hit
CGTTGCTGAAAGTTGTATATAACTTTTTATAGGCACATAGGCCCATTGAT275040.1906288297826773No Hit
CGTTGTTAAAAGTTGTATTTCATTTAAAGGTCAGAGACCAGTAAACAGAC234240.16235055660374612No Hit
CGTTGTTGAAAGTTTTGATTTATTTGTTTTTTTACTCAGAAGTTCCACTA220560.15286901794963392No Hit
CGTTGTTAAAAGTTGTTTTATAGATTTTTTAGGTCTATGTTACAATATTA219500.1521343373229264No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTTCAATCTT212630.14737277514794456No Hit
CGTTGTTGAAAGTTATGTTTTAGAATTTAGTCAATTCTCTAACATTTTCA197150.13664366561829128No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTAATCTCAGACTACAATCTT181500.12579672995039243No Hit
CGTTGCTGAAAGTTTTATTAAACTTTTAATATTTCAACAGCTTAACTGCT174110.12067475841136543No Hit
CGTTGTTGAAAGTTTTGATTCATTTGTTTTACGGGCTTTCGCCCCTCAGA171440.11882419494597948No Hit
CGTTGTTGAAAGTTGTAACTATTATGTTTTTTCAGACGCTGATTGCAACT167110.11582309389537232No Hit
CGTTGTTGAAAGTTTTATTATGTTATTAAAACGTTTACATTCATAAGACT144330.10003439137046907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAGT10249350.0244.69767
TTGAAAG10121600.0244.633266
GAAAGTT10251000.0244.511228
CGTTGTT10341050.0243.280611
GTTGAAA10186550.0242.609625
TGTTGAA10239550.0242.25594
GTTGTTG10197200.0242.062292
TTGTTGA10324100.0240.796393
AAAGTTT9491000.0240.716029
GCTGAAA151000.0229.910895
CTGAAAG154050.0226.313966
ACAAGCT246550.0223.83344245
GGAGATT113800.0212.815169
AGGAGAT112900.0208.109948
GGAGGAG125150.0198.800386
CGTTGGG150700.0188.586181
ACAAGCC649400.0186.63573245
TGAACGT53800.0186.483237
TGCAAGT49300.0175.67537
TAAAAGT389050.0173.840327
Produced by FastQC (version 0.11.5)