000000000-BRWHK_l01.2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-BRWHK_l01.2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14428038
Sequences flagged as poor quality	0
Sequence length	12
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTTCGGTAGAT	1013853	7.026963749333069	No Hit
AGAGCCTACGTT	413595	2.866605979274521	No Hit
CCATGCGATAAC	398564	2.762426880217532	No Hit
AGCCGGCACATA	390484	2.7064248098043544	No Hit
TGCAATGTTGCT	380303	2.635860814893889	No Hit
CTACCGGATCAA	368314	2.5527656636335445	No Hit
GCTCAGTGCAGA	281372	1.950175068848585	No Hit
GTCTACACACAT	233418	1.6178083257058236	No Hit
AGCTTGACAGCT	219981	1.524677159846682	No Hit
CGTCCGAAATAC	212667	1.4739841966038625	No Hit
TAAACCGCGTGT	207801	1.4402581972684019	No Hit
CGACTGTCTTAA	199505	1.3827590418045752	No Hit
TCGTCGATAATC	193254	1.3394336776767568	No Hit
TACTTCGCTCGC	188342	1.3053888546731025	No Hit
GGTCAGCTTAAC	187352	1.2985272148576266	No Hit
TATGCACCAGTG	181255	1.2562692169233267	No Hit
TGGCACCGATTA	171816	1.190847986399814	No Hit
TCTGTTGCTCTC	168985	1.1712264689072762	No Hit
AGCAAACACCCG	168279	1.1663332186954318	No Hit
CCATAGGGTTCA	154651	1.0718782415183548	No Hit
TCTACGGAGAGC	153439	1.0634779309563782	No Hit
AACGAGAACTGA	150067	1.0401067698879085	No Hit
GCTCGAAGATCG	145880	1.0110868851329613	No Hit
TATCAGGTGTGC	129359	0.8965806716062156	No Hit
AACACAAGGAGT	123887	0.8586545169897668	No Hit
CTCTACCTCTAC	123471	0.8557712420774052	No Hit
GCAGGATAGATA	122444	0.8486531571375124	No Hit
TCTTCCGCTACT	119385	0.8274513832026227	No Hit
ACGGCATGGCAT	118681	0.8225719948893953	No Hit
AATCTTGCTGCA	110331	0.7646985681629062	No Hit
TAACACCACATC	108903	0.7548011725502802	No Hit
CTTACACCAAGT	103055	0.7142689809938122	No Hit
GTCCGAAACACT	101527	0.7036784904503302	No Hit
ACGATGCGACCA	100803	0.6986604831509315	No Hit
CGGACTACAACT	98123	0.6800855390039866	No Hit
TAACGCTTGGGT	96854	0.6712901643314219	No Hit
AATGTCCGTGAC	95391	0.6611501854929963	No Hit
ACTCACGGTATG	92573	0.6416187703414699	No Hit
AAGATGGATCAG	91505	0.6342165164799262	No Hit
TCGGAGTGTTGT	89350	0.619280320719976	No Hit
GACACATTTCTG	87527	0.6066452001304682	No Hit
ACCACATACATC	81932	0.5678665387490662	No Hit
CTCGAGAGTACG	81590	0.5654961540855381	No Hit
TGGCATACGGCA	79260	0.5493470421965897	No Hit
ACAAGGAGGTGA	79151	0.5485915687219565	No Hit
TCAAGCTCAAGC	71007	0.4921459175530311	No Hit
CACATCTAACAC	68591	0.4754007440235464	No Hit
CCATACATAGCT	60649	0.42035514461495044	No Hit
GTACCTAATTGC	60481	0.41919074513111204	No Hit
CTAGATTTGCCA	59031	0.4091408686336978	No Hit
ATTGGGCTAGGC	55031	0.3814170713994515	No Hit
AGTTCCCGAGTA	54560	0.37815259427511905	No Hit
AGCCTAAGCACG	53683	0.37207415173151054	No Hit
ATGATGACCCGT	51913	0.3598063714553566	No Hit
CAGGAAGGTTAA	51874	0.35953606443232267	No Hit
CCTAGTACTGAT	51416	0.3563616896490015	No Hit
TTGATGCTATGC	50307	0.3486752668658067	No Hit
TTATGCAGTCGT	47273	0.3276467666636309	No Hit
TAATCCACAGCG	45674	0.316564178719241	No Hit
TACTAATCTGCG	44877	0.3110402121203174	No Hit
GGTCACTGACAG	43736	0.3031319989592487	No Hit
ATGGTTGTTGGC	41124	0.28502835936528587	No Hit
TCAACAGCATCG	40175	0.27845088847146093	No Hit
ACACTAGATCCG	34233	0.23726718767998808	No Hit
GCATGGCTCTAA	33169	0.22989265761567856	No Hit
TGTTATCGCACA	32248	0.22350925330249338	No Hit
ATTAGTTCGCGT	30557	0.2117890180217158	No Hit
CAACACGCACGA	30299	0.2100008331001069	No Hit
TGTAATTGTCGC	27768	0.19245860040013757	No Hit
ATGGACCGAACC	26428	0.18317112832666507	No Hit
TAGCGGATCACG	23121	0.16025047896325198	No Hit
ATGTGGGACCCA	22684	0.15722165411541056	No Hit
GTGGGATGTTTC	22385	0.15514930027215065	No Hit
TGGCAAGACTCT	21676	0.1502352572123805	No Hit
AATGGAGCATGA	20173	0.13981804040161247	No Hit
GTATTACGATCC	19706	0.13658128707451422	No Hit
TGACCTCCAAGA	19171	0.13287322919443378	No Hit
TAAGGTAAGGTG	17628	0.12217877441132328	No Hit
CCAAGTCTTACA	16805	0.11647460313037711	No Hit
GATTGGTTGCAC	14959	0.10368007070677247	No Hit
ATGTCGAGAGAA	14952	0.10363155406161254	No Hit

Adapter Content

Can't analyse adapters as read length is too short

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAGC	250	0.0	6.0	6
CTATCGG	240	0.0	6.0	1
GTGGGAC	2295	0.0	6.0	3
AGCCAGC	55	0.009160594	6.0	1
ACCTGTG	105	4.830599E-7	6.0	6
TCGGCCT	55	0.009160594	6.0	1
TCGGCAG	250	0.0	6.0	4
ACGATGG	120	2.5693225E-8	6.0	1
GGGACCC	2315	0.0	6.0	5
TCGTCGG	190	0.0	6.0	1
GGACGGT	65	0.0012619485	6.0	3
GATGCGC	55	0.009160594	6.0	3
GCAGGCT	110	1.8152605E-7	6.0	1
CCGCGGG	65	0.0012619485	6.0	5
GACTGTC	20435	0.0	6.0	2
CGCCGGC	250	0.0	6.0	1
CGTCCGG	55	0.009160594	6.0	1
AGGGCCT	80	6.539213E-5	6.0	1
GCGTCGA	60	0.003396516	6.0	1
AGTCGGC	135	1.375156E-9	6.0	1

Summary