FastQCFastQC Report
Mon 22 Oct 2018
000000000-BRWHK_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BRWHK_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14428038
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTGCCAGCGCTTA228062515.806896266838223No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCC208222414.431788993070299No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAA9036346.263041447492721No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCAGAGTGCCCTAGGCTCTCCAA8135685.638798567067816No Hit
GTTGGTGAACCAGCGGAGGGATCATTACAGAGTTATCCAACTCCCAAACC5122353.5502748190710336No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGGTCC4265302.956257808580765No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTT2542891.762464168724812No Hit
GTAGGTGAACCTGCGGAAGGATCACTAGAGATATCGCCCTTCGGGGCTCT2361101.6364664412444714No Hit
GTAGGTGAACCTGCGGAGGGATCATTACACAACAAAATATGAAGGCCCTG1594041.1048210435819479No Hit
GTAGGTGAACCTGCGGAAGGATCACTAGAGATTTCGCCCTCCGGGGCTCT1556201.0785943313983508No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTGACCGTCTGTCGAGCTT1152780.7989859743923602No Hit
GTAGGTGAACCTGCGGAAGGATCATAAAGAAGCTTCGGCGCTAGCCGGAG1094890.7588627088450973No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACCACC1057840.7331835416568767No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAGAACACCGGTGTAAAAGCC837530.5804877974399569No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAACTTA740630.5133268986399953No Hit
GTTGGTGAACCAGCGGAGGGATCATTGCTGGAACGCGCCCCAGGCGCACC717360.49719857959897246No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG686510.47581660098206No Hit
GTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCTGGA600270.41604409414502513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG576580.3996246752330428No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCC558690.38722520692002615No Hit
GTAGGTGAACCTGCGGAAGGATCATTATAGTATTCTATTGCCAGCGCTTA530950.3679987535380764No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTGTGGACTTCGGTC463540.32127722424906285No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAGTTATTGAGAGTAGCTCTG413650.28669871814864917No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTTTGGGTCC409420.2837669265911276No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATATTCTTGATGGGTTGTA400480.2775706579092736No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGTAAAA347420.24079504087804593No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCGTGGCCC270650.1875861430362188No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTAGGGTTCCTAGCGAG266210.18450880154321747No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAAAGTAAAATGTAGTTGGTT254370.17630255756188054No Hit
GTAGGTGAACCTGCGGAGGGATCATTACACAACAAAATATGAAGGCCTGG215060.14905699582992504No Hit
GTAGGTGAACCTGCGGAAGGATCATTCACAATAAGAGTGTTTATGGCACT209690.14533507605122747No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAAATTTAATAATTTTGAAAA178360.1236204118675041No Hit
GTAGGTGAACCTGCGGAAGGATCACTAGTGATTAAATCGAGCGTGTCTTC171320.11874102355427674No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTGTAGACTTCGGTC170800.11838061419023156No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCAGAGTGCCTTTTGGCTCTCCA166110.11512999896451617No Hit
GTAGGTGAACCTGCGGAAGGATCACTAAAGAATTCGCCCTTCGGGGCTCT157740.10932879439325015No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAATAATCAATAATTTTGGCTTGT153070.10609204106615189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTGA6804600.0244.790952
TTGGTGA4051650.0244.292332
ACCTGCG6911250.0244.191579
GAACCTG6912200.0244.150917
ACCAGCG4028950.0243.658959
TGAACCT6928600.0243.495216
GGTGAAC10938500.0243.467774
AGGTGAA6858250.0243.304723
TGAACCA4031200.0243.261616
GTGGAGT977550.0242.94467245
GTGAACC10972950.0242.8055
GTAGGTG6861850.0242.800381
GTTGGTG4100750.0241.606281
TGGTGAA4114600.0241.224723
AACCAGC4094800.0239.614938
GAACCAG4097350.0239.44197
AACCTGC7088950.0238.099758
CCGAGCA142750.0215.13643245
CCGACGG119650.0196.26604245
CCGTGCA373700.0193.9609245
Produced by FastQC (version 0.11.5)