FastQCFastQC Report
Wed 12 Dec 2018
000000000-BPJBB_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BPJBB_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041970
Sequences flagged as poor quality0
Sequence length251
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA354171.7344525139938394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG309571.5160359848577598No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC58530.28663496525414184TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC45510.2228730098875106TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC36820.18031606732713995TruSeq Adapter, Index 12 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG203050.034.3153233
GATCGGA206700.034.001351
ATCGGAA207800.033.7234462
CGGAAGA234600.030.1544574
AGAGCAC235100.029.7776328
GAGCACA235000.029.7619329
GAAGAGC240400.029.510146
GGAAGAG263200.027.1817825
AAGAGCA272000.026.0082197
GAATCGG3855.0931703E-1122.5153481
CCTTGTA12150.021.29350930-34
TAGGCCG2200.018.7905545-49
CACCTTG18000.018.52205830-34
ACCTTGT13950.018.35476330-34
GTCACCT17450.018.26420825-29
TGTAATC11850.017.89354135-39
GTAATCT12800.017.60735935-39
AATCGGA5806.8394E-1016.09442
TCACCTT19500.016.0030630-34
TAATCTC14250.016.00290335-39
Produced by FastQC (version 0.11.5)