FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686540
Sequences flagged as poor quality0
Sequence length41
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAA76220.4519311726967638No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAA71600.424537811139967No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAA64090.38000877536257666No Hit
GATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAA55290.32783094382582095No Hit
GATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAA53040.3144900209897186No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAA50270.2980658626537171No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAA46670.27672038611595334No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAA45620.2704946221257723No Hit
GATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAA42000.2490305596072432No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAA33490.19857222479158515No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAA32370.1919314098687253No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAA27340.16210703570623883No Hit
ATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAA26530.15730430348524196No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAA24740.1466908582067428No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAA24120.14301469280301682No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAA23980.14218459093765934No Hit
ATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAA23040.13661104984168773No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAA20460.12131345832295706No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAAA18490.10963273921756969No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAA17750.1052450579292516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAA251.710822E-435.0000047
CCCGTAT251.710822E-435.00000431
AACATCG251.710822E-435.00000413
CCCGGAA251.710822E-435.00000428
ATTTACA200.00241975335.033
TAAAGTA200.00241975335.029
ATAATTG301.2168832E-535.04
GAATAAT358.7031367E-735.02
AGAGTTC200.00241975335.030
ACGATCG301.2168832E-535.01
GTTAAGA358.7031367E-735.09
AAGTTCT200.00241975335.031
GGGAATC358.7031367E-735.031
AGACCCG200.00241975335.028
TGTACTC301.2168832E-535.032
ACCCCGA200.00241975335.014
GAAGGTC406.254231E-835.05
CCCATTA9200.034.42934827
ACCCGAA7950.034.33962230
GACCCGA8150.034.14110629
Produced by FastQC (version 0.11.5)