FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n02_Cas9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n02_Cas9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4631551
Sequences flagged as poor quality0
Sequence length41
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAA147763731.903718646302288No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAA137267329.637436789533357No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAA128390627.7208649974922No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGTATTCAA64130.138463335500354No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGTATTCAA62810.13561331830309112No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGTATTCAA54570.11782230185957146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTCG900.035.00000416
TTAGAAG200.00242018735.00000431
AGCCCGT406.258233E-835.00000425
CTCGAAT1350.035.00000431
CTACACT200.00242018735.0000041
CGACAAG200.00242018735.00000425
AGCCTAG1300.035.00000415
TAGCGCT1050.035.00000424
TAGCGCG454.5165507E-935.00000424
AAGTACT200.00242018735.00000414
CTTTATA200.00242018735.00000429
GCCTTAG200.00242018735.00000416
CGATACA650.035.0000041
GAAACTC650.035.0000042
GATACTA1350.035.0000042
AATTCTA406.258233E-835.00000435
TGTCCTA200.00242018735.00000415
GCTAGGC406.258233E-835.00000422
CGCCCAG200.00242018735.00000425
CAACCTA406.258233E-835.00000413
Produced by FastQC (version 0.11.5)