FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n02_CD55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n02_CD55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4848801
Sequences flagged as poor quality0
Sequence length41
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAA59293012.228383882943433No Hit
GATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAA57697911.899415958708143No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAA56377111.627018720710543No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAA52891110.908078100132384No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAA52752510.87949371401301No Hit
GATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAA4765629.828450373607826No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAA3829287.897375041788682No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAA3439757.094021800440975No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAA3071206.3339369877212945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAA950.035.00000424
GTTGAAT358.7076114E-735.00000430
CGGGTTT358.7076114E-735.00000430
CAGAATT700.035.00000431
TAGAGAC358.7076114E-735.00000423
AGCAAAT358.7076114E-735.00000424
AGTCATA358.7076114E-735.00000424
CAAAAGA358.7076114E-735.00000428
GTTTCAA358.7076114E-735.0000048
CACATGT358.7076114E-735.00000428
CACATGG358.7076114E-735.00000426
AGCTTCG358.7076114E-735.00000424
GATCCCC1100.035.0000041
ATAGCCT358.7076114E-735.00000422
TCGGGAA552.3646862E-1135.00000429
GCACGCG358.7076114E-735.00000425
CTAAGTA358.7076114E-735.00000417
CACTGTA358.7076114E-735.00000426
GAATACC358.7076114E-735.00000433
GAGAGCC552.3646862E-1135.00000421
Produced by FastQC (version 0.11.5)