FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n02_CD46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n02_CD46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4661615
Sequences flagged as poor quality0
Sequence length41
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAA57787712.39649778027572No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAA52631911.29048623706591No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAA52432011.247604102869929No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAA50901610.919305862882284No Hit
ATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAA49809310.684987928003492No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAA4502449.658541085010237No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAA3840728.239033038978981No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAA3651567.833250922695247No Hit
ATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAA3124296.70216223347488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTCA358.7075205E-735.024
GCCCCAA200.002420189335.024
GTAGATT601.8189894E-1235.029
GCCCTAT200.002420189335.024
CTCGAAT251.7112841E-435.029
AGCCCCC1000.035.023
TAGAACA200.002420189335.022
CAAGATC1400.035.011
CGACAAG200.002420189335.026
CGACAAC200.002420189335.025
AGCCTAT650.035.023
TTTGAAA200.002420189335.029
TTTTGAA251.7112841E-435.028
ACTCGTT200.002420189335.03
ACACGGC251.7112841E-435.026
CAAGACA2450.035.011
AACGCAT1850.035.029
ACTCGGG251.7112841E-435.026
CTATATG552.3646862E-1135.026
GGGCATT3100.035.030
Produced by FastQC (version 0.11.5)