FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n02_CD45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n02_CD45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001961
Sequences flagged as poor quality0
Sequence length41
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAAA58020114.497917395996613No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAAA44410811.097259568496543No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAAA3646839.11260754415148No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAA3597138.988418427865739No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAA3354448.381990729045086No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAA3161627.9001769382560205No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAAA3083757.705597330908522No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAA2954517.382655653066085No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAA2566006.411856587308072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAA301.2172997E-535.00000422
ACAACGA552.3646862E-1135.00000426
TTCTAGT251.7112408E-435.0000048
GAAATCC301.2172997E-535.00000431
CGGGTAT301.2172997E-535.00000428
TAATACT251.7112408E-435.0000041
TAGAGCT251.7112408E-435.00000421
ACACGTG601.8189894E-1235.00000425
CCGGGGA251.7112408E-435.00000427
GCCCAAA251.7112408E-435.00000423
ACACGGT251.7112408E-435.00000425
CGAACAT1000.035.00000424
GCAAGAC503.274181E-1035.0000049
ATTAGTC251.7112408E-435.00000430
CTACTTG251.7112408E-435.00000425
GCGACAA1000.035.00000423
TCACCAA301.2172997E-535.00000435
ACGCATT1100.035.00000429
CCGATAG251.7112408E-435.00000417
AACCCCG251.7112408E-435.00000413
Produced by FastQC (version 0.11.5)