FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686540
Sequences flagged as poor quality0
Sequence length71
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATGA254.411884E-665.0000163
TCGCATC9700.065.07
GATCCAT150.00298498365.047
ACTTAGG150.00298498365.050
CGTTAGC150.00298498365.033
TCTCGTA150.00298498365.039
TAAGCTT150.00298498365.053
GGTATTC16850.064.8071212
GATCTCT15700.064.7937
CACGATC15500.064.790334
ACGATCT15500.064.790335
ATGATCG15200.064.786196
ATCGTCT15200.064.786199
TAACGCA14100.064.769517
AACGCAT14100.064.769518
ATCGCGC13500.064.7592542
GATCGCG13450.064.758361
ATCGATT13050.064.750951
ACGATCG11300.064.712391
ATGGTCT9950.064.673376
Produced by FastQC (version 0.11.5)