FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n01_Cas9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n01_Cas9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4631551
Sequences flagged as poor quality0
Sequence length71
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGTTTAAGAGCTATGC65300.14098948710701878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCA150.002985311765.0000149
TCCCCCG150.002985311765.0000148
CTATCGA2650.065.02
CACTCGA201.1446458E-465.032
TATCGAT2650.065.03
ACGATCG1580250.064.98561
TCGATCG1374150.064.8770141
GATCGAT1584100.064.860493
ATCGATA1587100.064.838234
CGATAGG1579600.064.829236
TCGTTAC1371350.064.798565
ATCGTTA1374950.064.7896354
TCGATAG1585300.064.788845
TAAGTAG1469800.064.776671
TAGAGTC1466250.064.776135
AAGTAGA1469550.064.761152
AGCTATC68250.064.7142965
GATAGGT1584900.064.712917
CGATCGA1587300.064.688782
GTAGAGT1469850.064.679394
Produced by FastQC (version 0.11.5)