FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n01_CD55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n01_CD55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4848801
Sequences flagged as poor quality0
Sequence length71
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGC150.0029853265.000014
TACACTA1550.065.000012
TCGAACA150.0029853265.000014
AGATCGC900.065.04
TCGAATC201.1446508E-465.07
GATCGCG509650.064.9744951
TACACGA624200.064.968762
ATCGCGC509500.064.9234542
TCGATTC568950.064.885762
CACGATC625050.064.880414
AGGTCGC374000.064.869654
TCGCATC375700.064.8615957
ATGATCG651200.064.845286
GATCTCT626550.064.844397
GTATCTC340850.064.818836
CATGATC651850.064.805565
TCCTTGG565800.064.80477
ATCATGA652700.064.790873
GATCGTC651350.064.775478
ATCGATT569900.064.766181
Produced by FastQC (version 0.11.5)