FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n01_CD46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n01_CD46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4661615
Sequences flagged as poor quality0
Sequence length71
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGATC356.679329E-965.000015
ACGACCT356.679329E-965.000015
AGATCGC650.065.04
CTATCGA750.065.01
TACCGTG150.00298531365.051
GATCGCG389100.064.983291
TACACGA408700.064.976142
ATCGCGC389300.064.933212
ACTGTAT542850.064.8922353
TCGATTC571600.064.891972
ATACACG409250.064.8570561
GTATCTC541650.064.856
TCGCATC329050.064.841977
ATACAGG536900.064.8365557
ATGATCG484900.064.825746
ATAACGC566400.064.8106466
CATGATC485200.064.799055
TATCTCT545900.064.7856757
CACGATC409500.064.777784
TCCTTGG569850.064.777577
Produced by FastQC (version 0.11.5)