FastQCFastQC Report
Fri 22 Mar 2019
000000000-BNL8M_l01_n01_CD45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNL8M_l01_n01_CD45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001961
Sequences flagged as poor quality0
Sequence length71
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACC451.0913936E-1165.07
AGATCGC950.065.04
CTATACA800.065.05
TTCACGA201.14462666E-465.02
TCGATGC201.14462666E-465.04
TACAGGC254.412992E-665.08
GATCGCG313750.064.989641
TACACGA384800.064.974662
ATCGCGC313850.064.948222
TCGATTC278250.064.9182362
ACGATCG391450.064.916971
ACTGTAT634500.064.882193
CACGATC385650.064.8567354
ACACGAT386000.064.840033
TCGCATC476750.064.8295757
CGATAGG390000.064.808336
CATGATC343950.064.7732245
GATCTCT387450.064.765137
AACGCAT359550.064.7649848
GATCGTC343000.064.753658
Produced by FastQC (version 0.11.5)