FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_mldna_neblib_nebset1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_mldna_neblib_nebset1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059822
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC3952.6720954E-812.40506443
CGCCGTA3309.072918E-510.60606146
GCCGTAT4005.9298516E-59.62547
TGGTCGC5502.5352947E-68.90909142
GTCGCCG8101.2604505E-77.777777744
TCGCCGT9201.0531312E-66.84782645
CGTGTAG10701.8791106E-66.214953470
GAGCGTC13406.644768E-86.007462570
GTCGTGT12305.2047835E-75.9756168
CGTCGTG12952.1621054E-75.945945712
GAGTGTA13052.5113695E-75.90038327
AGCGTCG11759.203639E-65.659574510
GTAGGGA13001.4548059E-65.653846318
CCCGGGC7550.0078559045.56291445
GTGGTCG10758.3471765E-55.534883541
TCGTGTA14704.3040018E-75.476190669
GTGTAGA14101.1194661E-65.460993329
AGTGTAG12958.126193E-65.405405528
GCGTCGT12602.9584131E-55.277777766
CTCGGTG9959.939229E-45.27638237
Produced by FastQC (version 0.11.5)