FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_mldna_neblib_nebset1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_mldna_neblib_nebset1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059822
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC4151.8189894E-1114.3373543
CGCCGTA3458.7498665E-712.17391346
TGGTCGC5301.0695658E-911.22641542
GCCGTAT4005.1773986E-610.547
GTCGCCG8403.4015102E-108.7544
GACTGCG3650.00267103278.630136543
TCGCCGT8806.2935214E-87.55681845
GTGGTCG10602.2898712E-76.60377341
GAGTGTA13154.526555E-86.12167327
CGTGTAG11003.0126994E-66.045454515
GTAGGGA13353.904188E-75.76779118
CCCGGGC7400.00629478655.67567645
GAGCGTC14202.149227E-75.669014570
CGTCGTG13706.437749E-75.620437612
AGTGTAG13252.0671323E-65.547169728
AGCGTCG12201.7285613E-55.4508210
GTGTAGG14552.0405478E-65.292096616
GTGTAGA13904.970996E-65.2877729
CTCGGTG10000.00106269355.2537
TCGGTGG9400.00279163875.21276638
Produced by FastQC (version 0.11.5)