FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_mldna_neblib_dapv2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_mldna_neblib_dapv2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250834
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC3150.033.33333655
TGGTCGC3650.028.76712254
CGCCGTA3350.026.11940258
GCCGTAT3600.022.3611159
TGTAGGC7100.017.25352130
CCGTATC4950.016.96969860
TAGGCTA6800.016.47058932
GCTATAG7200.016.04166635
GTCGCCG7000.016.056
AGGCTAT7250.015.93103533
GGCTATA8050.015.21739234
GGTGGTC7650.015.0980452
TCGGTGG7300.014.86301350
GTAGGCT8150.014.1717831
CTCGGTG7250.014.049
TATAGTG9250.013.62162237
GATCTCG7800.013.0128246
CGTATCA6800.012.86764861
GTGGTCG9250.012.86486553
CTATAGT9150.012.62295136
Produced by FastQC (version 0.11.5)