FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_mldna_neblib_dapv2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_mldna_neblib_dapv2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250834
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC3050.032.1311555
TGGTCGC3500.028.054
CGCCGTA3150.026.66666858
GCCGTAT3550.021.69014259
CCGTATC4800.016.77083260
TGTAGGC7000.016.530
GTCGCCG6800.015.44117756
TAGGCTA6950.015.10791332
GCTATAG7250.014.96551735
AGGCTAT7500.014.46666733
GGCTATA7750.014.45161234
GGTGGTC7550.014.37086152
CTCGGTG6600.014.31818249
TCGGTGG7150.014.19580450
GTAGGCT7800.013.91025631
GATCTCG7500.013.06666846
TCGCCGT7000.012.557
GTGGTCG9150.012.240437553
TATAGTG9250.012.108108537
CGTATCA6950.012.0863361
Produced by FastQC (version 0.11.5)