FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_mldna_daplib_nebset1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_mldna_daplib_nebset1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630351
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC1656.5240846E-414.84848521
GGGTGCA3150.00780404178.88888845
CGTCGTG12101.8189894E-117.520661470
GCGTCGT11951.10958354E-107.32217669
AGCGTCG12908.54925E-117.054263670
GAGCGTC13602.928573E-106.69117770
AGAGCGT16251.8189894E-126.676923369
TGACCCT5250.00903288656.666666522
CCCATGT6250.0069714916.1659
CGTGTAG8003.5992987E-46.125000570
GTCGTGT10606.742984E-55.61320870
CAGATCG12058.237797E-55.22821669
Produced by FastQC (version 0.11.5)