FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_mldna_daplib_nebset1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_mldna_daplib_nebset1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736489
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCGA2300.00789429910.6521748
GGGACCC4950.00511213257.07070718
CTTTACG9207.6725155E-66.4673911
CTCGGTG7750.00163622265.87096870
GCAACCG14453.0517367E-75.570934316
TATGACT63800.05.43103462
CTATAGC9050.00173338275.41436431
GAGTGTA18802.761226E-95.398936367
ATTATCG11755.5345547E-55.3617021
TGTCATG49800.05.341365312
ATATATG53700.05.27932931
CCCGATC22001.7462298E-105.2551
GATGTCA43050.05.20325210
CTCCTCT22202.237357E-105.2027031
AGTGTAG18901.762237E-85.185185468
GTCATGT48100.05.166320313
ATATGAC61000.05.1639341
CTATGAA26551.8189894E-125.14124351
GTAGGGA30700.05.13029368
CTATTAA27601.8189894E-125.07246351
Produced by FastQC (version 0.11.5)