FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_abdna_neblib_nebset1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_abdna_neblib_nebset1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558946
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2950.022.54237443
TGGTCGC3750.019.642
CGCCGTA2203.5088306E-919.0909146
GAGTGTA9150.014.5355227
AGTGTAG9300.012.79569928
GCCGTAT3356.1261926E-712.53731347
CTCGGTG6750.012.44444537
GTAGGGA10500.012.018
AGAGTGT11300.011.76991226
GGTGGTC7750.011.74193640
TCGGTGG7200.011.66666738
GATCTCG7050.011.41843934
CCGTATC4752.8940121E-811.05263148
TGTAGGG11150.010.986546517
GTCGCCG6151.0004442E-1010.81300844
CGTGTAG12050.010.74688815
GTGGTCG8250.010.60606141
GTGTAGA10950.010.54794629
ATCTCGG10050.010.09950235
GTCGTGT12650.09.96047413
Produced by FastQC (version 0.11.5)