FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_abdna_neblib_nebset1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_abdna_neblib_nebset1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558946
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2151.1459633E-1021.1627946
GGTCGCC2850.020.87719243
TGGTCGC3550.018.73239342
GAGTGTA9450.014.44444527
CTCGGTG6850.013.28467137
AGTGTAG9550.013.19371728
GCCGTAT3651.278604E-712.46575447
TCGGTGG7200.012.15277938
GATCTCG7250.012.06896634
GTAGGGA10750.012.04651218
AGAGTGT11350.012.02643226
CCGTATC4752.2992026E-911.78947448
TGTAGGG11500.010.95652117
GGTGGTC7700.010.90909140
CGTGTAG12200.010.9016415
GTGTAGA11150.010.67264529
ATCTCGG10200.010.63725535
GTCGTGT12500.010.3613
GTAGATC10550.010.2843631
GTGGTCG8550.010.23391841
Produced by FastQC (version 0.11.5)