FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_abdna_neblib_dapv2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_abdna_neblib_dapv2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994192
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2550.038.4313755
TGGTCGC2750.034.36363654
CGCCGTA2150.034.18604758
GCCGTAT1850.034.05405459
TGTAGGC3900.027.82051330
GCTATAG4050.026.79012335
TATAGTG4150.026.14457737
CTCGGTG3750.026.13333549
CCGTATC2550.026.0784360
TAGGCTA4050.025.92592632
TCGGTGG4100.025.60975650
GTAGGCT4500.024.1111131
AGGCTAT4400.023.86363633
GTCGCCG4100.023.0487856
GGTGGTC4150.022.77108252
GAGTGTA5100.022.64705727
CTATAGT4650.022.58064736
CGCCGCA952.781041E-422.10526358
GTGTAGA4850.020.92783541
ATAGTGT5500.020.36363638
Produced by FastQC (version 0.11.5)