FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_abdna_neblib_dapv2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_abdna_neblib_dapv2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994192
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2600.039.03846455
CGCCGTA2150.035.81395358
TGGTCGC2750.035.63636454
GCCGTAT1900.035.059
TGTAGGC3950.028.35443130
GCTATAG4100.027.31707435
CTCGGTG3750.027.06666849
CCGTATC2600.026.92307960
TAGGCTA4050.026.79012332
TATAGTG4300.026.04651337
TCGGTGG4200.025.83333450
GTAGGCT4450.025.1685431
AGGCTAT4400.024.6590933
CTATAGT4850.023.09278336
GGTGGTC4250.023.05882352
GAGTGTA5250.022.66666627
GTCGCCG4350.022.52873656
CGCCGCA952.781041E-422.10526358
GTGTAGA4950.021.2121241
GTGGTCG5250.020.66666653
Produced by FastQC (version 0.11.5)